Rabbit Anti-Alkaline Phosphatase, Tissue Non-Specific isozyme antibody

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AKP2; Alkaline phosphatase liver/bone/kidney; Alkaline phosphatase liver/bone/kidney isozyme; Alkaline phosphatase tissue nonspecific isozyme; Alkaline phosphatase, tissue-nonspecific isozyme; ALPL; AP TNAP; AP-TNAP; APTNAP; BALP; BAP; FLJ40094; FLJ93059;

Cat:

SL1535R

Species Reactivity：

Human,Mouse,(predicted: Rat,Dog,Pig,Cow,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human Alkaline Phosphatase, Tissue Non-Specific isozyme:52-150/524

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Placenta (Mouse) Lysate at 30 ugPrimary: Anti- Alkaline Phosphatase Tissue Non-Specific isozyme (SL1535R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilutionPredicted band size: 56 kDObserved band size: 60 kD

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.

Function:
This isozyme may play a role in skeletal mineralization.

Subunit:
Homodimer.

Subcellular Location:
Cell membrane; Lipid-anchor, GPI-anchor.

Post-translational modifications:
Glycosylated.

DISEASE:
Defects in ALPL are a cause of hypophosphatasia (HOPS) [MIM:146300]. HOPS is an inherited metabolic bone disease characterized by defective skeletal mineralization. Four hypophosphatasia forms are distinguished, depending on the age of onset: perinatal, infantile, childhood and adult type. The perinatal form is the most severe and is almost always fatal. Patients with only premature loss of deciduous teeth, but with no bone disease are regarded as having odontohypophosphatasia (odonto).
Defects in ALPL are a cause of hypophosphatasia childhood type (HOPSC) [MIM:241510].
Defects in ALPL are a cause of hypophosphatasia infantile type (HOPSI) [MIM:24300].

Similarity:
Belongs to the alkaline phosphatase family.

SWISS:
P09242

Gene ID:
249

Database links:

Entrez Gene: 249 Human

Entrez Gene: 11647 Mouse

Entrez Gene: 25586 Rat

Omim: 171760 Human

SwissProt: P05186 Human

SwissProt: P09242 Mouse

SwissProt: P08289 Rat

Unigene: 75431 Human

Unigene: 288186 Mouse

Unigene: 82764 Rat

ALP广泛分布于人体肝脏、骨骼、肠、肾和胎盘等组织,孕妇、骨折愈合期、骨软化症。佝偻病、骨细胞癌、骨质疏松、肝脓肿、肝结核、肝硬变、白血病、甲状腺机能亢进时，血清碱性磷酸酶亦可升高. Picture

Sample: Placenta (Mouse) Lysate at 30 ug
Primary: Anti- Alkaline Phosphatase Tissue Non-Specific isozyme (SL1535R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution
Predicted band size: 56 kD
Observed band size: 60 kD

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