Rabbit Anti-CCL18 antibody | Sunlong Medical

Home > Product > Antibody > Rabbit Anti-CCL18 antibody

Macrophage Inflammatory Protein 4; Alternative macrophage activation associated CC chemokine 1; Alternative macrophage activation-associated CC chemokine 1; AMAC-1; AMAC1; C-C motif chemokine 18; CC chemokine PARC; CCL18(1-68); CCL18(3-69); CCL18(4-69); C

Cat:

SL1989R

Species Reactivity：

Mouse,(predicted: Human,)

Immunogen：

KLH conjugated synthetic peptide derived from human MIP4/CCL18:21-89/89

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Paraformaldehyde-fixed, paraffin embedded (Mouse lung melanocytoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MIP4) Polyclonal Antibody, Unconjugated (SL1989R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse melanocytoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MIP4) Polyclonal Antibody, Unconjugated (SL1989R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (SL0295G-PE) for 90 minutes, and DAPI for nuclei staining.

prev next

Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

This chemotactic factor that attracts lymphocytes but not monocytes or granulocytes may be involved in B cell migration into B cell follicles in lymph nodes. It attracts naive T lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T cells, CD4+ and CD8+ T cells and thus may play a role in both humoral and cell-mediated immunity responses. It is expressed at high levels in lung, lymph nodes, placenta, and bone marrow, not expressed by peripheral blood monocytes, and a monocyte-to-macrophage differentiation is a prerequisite for expression. Specifically induced in macrophages by IL4, IL13, and IL10, it belongs to the intercrine beta (chemokine CC) family.

Function:
Chemotactic factor that attracts lymphocytes but not monocytes or granulocytes. May be involved in B-cell migration into B-cell follicles in lymph nodes. Attracts naive T-lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T-cells, CD4+ and CD8+ T-cells and thus may play a role in both humoral and cell-mediated immunity responses.

Subcellular Location:
Secreted.

Tissue Specificity:
Expressed at high levels in lung, lymph nodes, placenta, bone marrow, dendritic cells present in germinal centers and T-cell areas of secondary lymphoid organs and macrophages derived from peripheral blood monocytes. Not expressed by peripheral blood monocytes and a monocyte-to-macrophage differentiation is a prerequisite for expression. Expressed in synovial fluids from patients with rheumatoid and septic arthritis and in ovarian carcinoma ascitic fluid.

Similarity:
Belongs to the intercrine beta (chemokine CC) family.

SWISS:
P55774

Gene ID:
6362

Database links:

Entrez Gene: 6362 Human

Omim: 603757 Human

SwissProt: P55774 Human

Unigene: 143961 Human

Picture

Paraformaldehyde-fixed, paraffin embedded (Mouse melanocytoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MIP4) Polyclonal Antibody, Unconjugated (SL1989R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (SL0295G-PE) for 90 minutes, and DAPI for nuclei staining.

Product Feedback Wall