Rabbit Anti-Integrin Alpha V + Beta 1 antibody

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Integrin alpha 5 beta 1; fibronectin receptor alpha; ITGAV; CD51; DKFZp686A08142; MSK8; Msk 8; VNR; similar to Integrin alpha-V precursor; Vitronectin receptor subunit alpha; CD51 antigen; HGNC; Vitronectin receptor alpha polypeptide; Vitronectin receptor

Cat:

SL2016R

Species Reactivity：

Human,Rat,(predicted: Mouse,Dog,Pig,Cow,Horse,Sheep,)

Immunogen：

KLH conjugated synthetic Mixed peptides 915-948/1048(human Integrin αV)+ 21-70/798(human Integrin β1)

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500Flow-Cyt=1μg /test（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: A549 (Human) Cell Lysate at 30 ugLane 2: U251 (Human) Cell Lysate at 30 ugLane 3: Huvec (Human) Cell Lysate at 30 ugLane 4: SH-SY5Y (Human) Cell Lysate at 30 ugLane 5: U2os (Human) Cell Lysate at 30 ugLane 6: MCF-7 (Human) Cell Lysate at 30 ugLane 7: Jurkat (Human) Cell Lysate at 30 ugPrimary: Anti-Integrin Alpha V + Beta 1 (SL2016R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 125 kDObserved band size: 125 kDBlank control (blue line): MCF7 (fixed with 70% methanol overnight at 4℃). Primary Antibody (green line): Rabbit Anti-Integrin Alpha V + Beta 1 antibody (SL2016R), Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1μg /test.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells.
Integrin alpha 5 belongs to the integrin alpha chain family. It contains seven FG-GAP repeats.
Integrin beta 1 belongs to the integrin beta chain family. It contains one VWFA domain.
Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HISLV1 infection, the interaction with extracellular viral Tat protein se
ems to enhance angiogenesis in Kaposi's sarcoma lesions.

Function:
The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HISLV1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

Subcellular Location:
Membrane.

Similarity:
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.

SWISS:
P06756

Gene ID:
3685

Database links:

Entrez Gene: 3685 Human

SwissProt: P06756 Human

Picture

Blank control (blue line): MCF7 (fixed with 70% methanol overnight at 4℃).
Primary Antibody (green line): Rabbit Anti-Integrin Alpha V + Beta 1 antibody (SL2016R), Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1μg /test.

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