Rabbit Anti-KCNH1 antibody | Sunlong Medical

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EAG1; EAG 1; EAG-1; KCNH 1; KCNH-1; h eag; Kv10.1; MGC124420; MGC142269; EAG; Ether a go go potassium channel 1; hEAG1; M eag; MGC124419; Potassium voltage gated channel subfamily H (eag related), member 1; Voltage gated potassium channel subunit Kv10.1;

Cat:

SL2424R

Species Reactivity：

Human,Mouse,(predicted: Rat,Dog,Pig,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human KCNH1:501-600/989<Cytoplasmic>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Hippocampus (Mouse) Lysate at 40 ugPrimary: Anti- KCNH1 (SL2424R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 111 kDObserved band size: 111 kDBlank control: Hela(blue). Primary Antibody:Rabbit Anti-KCNH1 antibody(SL2424R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.ProtocolThe cells were fixed with 2% paraformaldehyde (10 min). Antibody (SL2442R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of SL2442R at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a member of the potassium channel, voltage-gated, subfamily H. This member is a pore-forming (alpha) subunit of a voltage-gated non-inactivating delayed rectifier potassium channel. It is activated at the onset of myoblast differentiation. The gene is highly expressed in brain and in myoblasts. Overexpression of the gene may confer a growth advantage to cancer cells and favor tumor cell proliferation. Alternative splicing of this gene results in two transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008].

Function:
Pore-forming (alpha) subunit of voltage-gated non-inactivating delayed rectifier potassium channel. Channel properties may be modulated by cAMP and subunit assembly. Mediates IK(NI) current in myoblasts.

Subunit:
The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming alpha subunits that can associate with modulating beta subunits. Heteromultimer with KCNH5/EAG2. Interacts with ALG10B (By similarity).

Subcellular Location:
Membrane; Multi-pass membrane protein.

Tissue Specificity:
Highly expressed in brain and in myoblasts at the onset of fusion, but not in other tissues. Detected in HeLa (cervical carcinoma), SH-SY5Y (neuroblastoma) and MCF-7 (epithelial tumor) cells, but not in normal epithelial cells.

Similarity:
Belongs to the potassium channel family. H (Eag) (TC 1.A.1.20) subfamily. Kv10.1/KCNH1 sub-subfamily.
Contains 1 cyclic nucleotide-binding domain.
Contains 1 PAC (PAS-associated SLCterminal) domain.
Contains 1 PAS (PER-ARNT-SIM) domain.

SWISS:
O95259

Gene ID:
3756

Database links:

Entrez Gene: 3756 Human

Entrez Gene: 16510 Mouse

Entrez Gene: 65198 Rat

Omim: 603305 Human

SwissProt: O95259 Human

SwissProt: Q60603 Mouse

SwissProt: Q63472 Rat

Unigene: 553187 Human

Unigene: 4489 Mouse

Unigene: 11071 Rat

KCNH1钾通道蛋白在调控细胞增殖和细胞周期进程中起重要作用。Eag1是膜蛋白, 参与细胞信号转导, 在正常组织和恶性肿瘤中有独特的表达特点.
一些离子通道如钾、钙、氯、钠等通道与肿瘤相关，特别是钾通道与肿瘤的关系最为密切。钾通道是细胞膜上种类最多、最为复杂的一类离子通道，它存在于多种细胞中，是细胞膜电位的重要控制因素。 Picture

Blank control: Hela(blue).
Primary Antibody:Rabbit Anti-KCNH1 antibody(SL2424R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Antibody (SL2442R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of SL2442R at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

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