CLEC2C; AIM; Early activation antigen CD69; Early T-cell activation antigen p60; GP32/28; Leukocyte surface antigen Leu-23; MLR-3; EA1; BL-AC/P26;C-type lectin domain family 2 member C; CD69_HUMAN;
Cat:
SL2499R
Species Reactivity：
Human,Mouse,(predicted: Rat,Dog,)
Immunogen：
KLH conjugated synthetic peptide derived from human CD69:101-199/199<Extracellular>
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-F=1:100-500Flow-Cyt=1μg/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Sample: U937 Cell Lysate at 30 ugPrimary: Anti- CD69 (SL2499R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 22 kDObserved band size: 34 kDSample: Lane 1: Blood cell (Mouse) Lysate at 40 ugLane 2: Spleen (Mouse) Lysate at 40 ugPrimary: Anti-CD69 (SL2499R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 33/27 kDObserved band size: 30 kDBlank control(blue): Molt-4 Cells(fixed with 2% paraformaldehyde (10 min)) . Primary Antibody: Rabbit Anti-CD69/FITC Conjugated antibody (SL2499R/FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.
This gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of the encoded protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. [provided by RefSeq, Aug 2011]
Function: Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.
Subunit: Homodimer; disulfide-linked.
Subcellular Location: Membrane; Single-pass type II membrane protein.
Tissue Specificity: Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets.
Post-translational modifications: Constitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes.
Sample: U937 Cell Lysate at 30 ug
Primary: Anti- CD69 (SL2499R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 34 kD
Sample:
Lane 1: Blood cell (Mouse) Lysate at 40 ug
Lane 2: Spleen (Mouse) Lysate at 40 ug
Primary: Anti-CD69 (SL2499R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33/27 kD
Observed band size: 30 kD
Blank control(blue): Molt-4 Cells(fixed with 2% paraformaldehyde (10 min)) . Primary Antibody: Rabbit Anti-CD69/FITC Conjugated antibody (SL2499R/FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.
Product Feedback Wall
Specific References (2) | SL2499R has been referenced in 2 publications.
[IF=12.121] Rouven Hoefflin. et al. HIF-1α and HIF-2α differently regulate tumour development and inflammation of clear cell renal cell carcinoma in mice. Nat Commun. 2020 Aug;11(1):1-21 IHC ; Mouse.
