Rabbit Anti-Versican antibody

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Versican; CSPG2; DKFZp686K06110; ERVR; GHAP; PG-M; WGN; WGN1; versican core protein isoform 1 precursor; ; Large fibroblast proteoglycan; Chondroitin sulfate proteoglycan 2; Chondroitin sulfate proteoglycan core protein 2; CSPG2; Glial hyaluronate binding

Cat:

SL2533R

Species Reactivity：

Mouse,Rat,(predicted: Human,Dog,Pig,Cow,Horse,)

Immunogen：

KLH conjugated synthetic peptide derived from human Versican:3301-3396/3396

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Versican) Polyclonal Antibody, Unconjugated (SL2533R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Versican) Polyclonal Antibody, Unconjugated (SL2533R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Versican) Polyclonal Antibody, Unconjugated (SL2533R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene is a member of the aggrecan/versican proteoglycan family. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and plays a central role in tissue morphogenesis and maintenance. Mutations in this gene are the cause of Wagner syndrome type 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Function:
May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid.

Subunit:
Interacts with FBLN1 (By similarity).

Subcellular Location:
Secreted, extracellular space, extracellular matrix.

Tissue Specificity:
Cerebral white matter and plasma. Isoform V0 and isoform V1 are expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas. Isoform V2 is restricted to normal brain and gliomas. Isoform V3 is found in all these tissues except medulloblastomas.

Post-translational modifications:
Phosphorylation sites are present in the extracellular medium.

DISEASE:
Defects in VCAN are the cause of Wagner syndrome type 1 (WGN1) [MIM:14640]. WGN is a dominantly inherited vitreoretinopathy characterized by an optically empty vitreous cavity with fibrillary condensations and a preretinal avascular membrane. Other optical features include progressive chorioretinal atrophy, perivascular sheating, subcapsular cataract and myopia. Systemic manifestations are absent in WGN.

Similarity:
Belongs to the aggrecan/versican proteoglycan family.
Contains 1 SLCtype lectin domain.
Contains 2 EGF-like domains.
Contains 1 Ig-like SLVtype (immunoglobulin-like) domain.
Contains 2 Link domains.
Contains 1 Sushi (CCP/SCR) domain.

SWISS:
P13611

Gene ID:
1462

Database links:

Entrez Gene: 1462 Human

Entrez Gene: 13003 Mouse

Entrez Gene: 114122 Rat

Omim: 118661 Human

SwissProt: P13611 Human

SwissProt: Q62059 Mouse

SwissProt: Q9ERB4 Rat

Unigene: 68761 Human

Unigene: 715773 Human

Unigene: 158700 Mouse

Unigene: 410783 Mouse

Unigene: 35666 Rat

Picture

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Versican) Polyclonal Antibody, Unconjugated (SL2533R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Versican) Polyclonal Antibody, Unconjugated (SL2533R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.

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