Rabbit Anti-IL21R antibody | Sunlong Medical

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IL 21R; IL-21R; IL21 Receptor; IL21R; Interleukin 21 receptor; MGC10967; NILR; IL21R_HUMAN; CD360; IL-21 receptor; IL21 Receptor; Interleukin-21 receptor; Novel interleukin receptor.

Cat:

SL2622R

Species Reactivity：

Mouse,(predicted: Human,Rat,Cow,)

Immunogen：

KLH conjugated synthetic peptide derived from human IL-21R:441-538/538

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample:Thymus (Mouse) Lysate at 40 ugPrimary: Anti- IL21R (SL2622R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 55 kDObserved band size: 55 kDSample:Lymph node (Mouse) Lysate at 40 ugPrimary: Anti- IL21R (SL2622R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 55 kDObserved band size: 55 kDTissue/cell:mouse lymphoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-IL21R Polyclonal Antibody, Unconjugated(SL2622R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this gene is a cytokine receptor for interleukin 21 (IL21). It belongs to the type I cytokine receptors, and has been shown to form a heterodimeric receptor complex with the common gamma-chain, a receptor subunit also shared by the receptors for interleukin 2, 4, 7, 9, and 15. This receptor transduces the growth promoting signal of IL21, and is important for the proliferation and differentiation of T cells, B cells, and natural killer (NK) cells. The ligand binding of this receptor leads to the activation of multiple downstream signaling molecules, including JAK1, JAK3, STAT1, and STAT3. Knockout studies of a similar gene in mouse suggest a role for this gene in regulating immunoglobulin production. Three alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2010]

Function:
This is a receptor for interleukin-21.

Subunit:
Heterodimer with the common gamma subunit. Associates with JAK1.

Subcellular Location:
Membrane; Single-pass type I membrane protein.

Tissue Specificity:
Selectively expressed in lymphoid tissues. Most highly expressed in thymus and spleen.

Post-translational modifications:
SLCmannosylated at Trp-214 in the WSXWS motif, the sugar chain makes extensive hydrogen bonds with Asn-73 sugar, and bridges the two fibronectin domains transforming the SLVshaped receptor into an A-frame.

Similarity:
Belongs to the type I cytokine receptor family. Type 4 subfamily.
Contains 2 fibronectin type-III domains.

SWISS:
Q9HBE5

Gene ID:
50615

Database links:

Entrez Gene: 50615 Human

Entrez Gene: 60504 Mouse

Entrez Gene: 308977 Rat

Omim: 605383 Human

SwissProt: Q9HBE5 Human

SwissProt: Q9JHX3 Mouse

SwissProt: Q5EBB1 Rat

Unigene: 210546 Human

Unigene: 155643 Mouse

Picture

Sample:
Lymph node (Mouse) Lysate at 40 ug
Primary: Anti- IL21R (SL2622R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD

Tissue/cell:mouse lymphoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-IL21R Polyclonal Antibody, Unconjugated(SL2622R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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