Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq, Jul 2008]
Function:
Involved in lymphocyte activation. Binds to HLA class-II antigens.
Subcellular Location:
Membrane; Single-pass type I membrane protein.
Tissue Specificity:
On cell surface of activated NK and T-lymphocytes.
Similarity:
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like SLVtype (immunoglobulin-like) domain.
SWISS:
Q5BK54
Gene ID:
3902
Database links:
UniProtKB/Swiss-Prot: P18627.5
Entrez Gene: 3902 Human
Omim: 153337 Human
SwissProt: P18627 Human
Unigene: 409523 Human
Picture |
Sample: K562 Cell (Human) Lysate at 40 ug
Primary: Anti-LAG-3 (SL2646R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 58 kD
Sample: 293T Cell (Human) Lysate at 40 ug
Primary: Anti-LAG-3 (SL2646R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 58 kD
Blank control:Ctll-2.
Primary Antibody (green line): Rabbit Anti-LAG-3 antibody (SL2646R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:Ctll-2.
Primary Antibody (green line): Rabbit Anti-LAG-3 antibody (SL2646R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
|
|
|