Rabbit Anti-PENK antibody | Sunlong Medical

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EP-ENK; Enkephalin A; P ENK; Preproencephalin A; Proenkephalin A; Proenkephalin; Synenkephalin; Proenkephalin; Opioid growth factor; OGF; PENK protein; ProenkephalinA; PENK_HUMAN; Met-enkephalin-Arg-Gly-Leu; Met-enkephalin-Arg-Phe; Synenkephalin; PENK(114

Cat:

SL2106R

Species Reactivity：

Mouse,Rat,(predicted: Human,Chicken,Dog,Horse,)

Immunogen：

KLH conjugated synthetic peptide derived from human PENK:150-161/267

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: NIH/3T3(Mouse) Cell Lysate at 30 ugPrimary: Anti-PENK (SL2106R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 29 kDObserved band size: 39 kDTissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Enkephalin/P-ENK Polyclonal Antibody, Unconjugated(SL2106R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include the pentapeptide opioids Met-enkephalin and Leu-enkephalin, which are stored in synaptic vesicles, then released into the synapse where they bind to mu- and delta-opioid receptors to modulate the perception of pain. Other non-opioid cleavage products may function in distinct biological activities. [provided by RefSeq, Jul 2015]

Function:
Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(237-258) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum.

Subcellular Location:
Secreted.

Post-translational modifications:
The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.

Similarity:
Belongs to the opioid neuropeptide precursor family.

SWISS:
P01210

Gene ID:
5179

Database links:

Entrez Gene: 5179 Human

Entrez Gene: 18619 Mouse

Entrez Gene: 29237 Rat

Omim: 131330 Human

SwissProt: P01210 Human

SwissProt: P4405 Mouse

SwissProt: P04094 Rat

Unigene: 339831 Human

Unigene: 475097 Mouse

Unigene: 10015 Rat

Picture

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Enkephalin/P-ENK Polyclonal Antibody, Unconjugated(SL2106R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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