The protein encoded by this gene is a membrane receptor for both human hepatitis A virus (HHAV) and TIMD4. The encoded protein may be involved in the moderation of asthma and allergic diseases. The reference genome represents an allele that retains a MTTVP amino acid segment that confers protection against atopy in HHAV seropositive individuals. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
Function:
May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair.
Subcellular Location:
Membrane; Single-pass type I membrane protein (Probable).
Tissue Specificity:
Widely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses.
Similarity:
Belongs to the immunoglobulin superfamily. TIM family. Contains 1 Ig-like SLVtype (immunoglobulin-like)
SWISS:
Q96D42
Gene ID:
26762
Database links:
Entrez Gene: 26762 Human
Entrez Gene: 171283 Mouse
Entrez Gene: 286934 Rat
Omim: 606518 Human
SwissProt: Q96D42 Human
SwissProt: Q5QNS5 Mouse
SwissProt: O54947 Rat
Unigene: 129711 Human
Unigene: 17771 Mouse
Unigene: 11154 Rat
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Sample: Brain(Mouse) lysate at 30ug;
Primary: Anti- HAVCR1c/TIM 1 (SL2713R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(SL0295G-HRP) at 1: 5000 dilution;
Predicted band size: 39 kD
Observed band size: 42 kD
Blank control: A549.
Primary Antibody (green line): Rabbit Anti-HAVCR1c antibody (SL2713R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 3μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:HepG2.
Primary Antibody (green line): Rabbit Anti-HAVCR1c/TIM 1 antibody (SL2713R)
Dilution: 1ug/Test;
Secondary Antibody (white blue line) : Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）：Normal Rabbit IgG
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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