Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Mouse B16 cell lysates
Lane 3: Human SH-SY5Y cell lysates
Lane 4: Human 293T cell lysates
Primary: Anti-CITED1 (SL2990R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 20 kDa
Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CITED1 ) Polyclonal Antibody, Unconjugated (SL2990R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.