Rabbit Anti-TNFAIP3 antibody

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Zinc finger protein A20; A20; TNFA1P2; TNFAIP 3; Tnfip 3; Tnfip3; Tumor necrosis factor alpha induced protein 3; Tumor necrosis factor induced protein 3; Tumor necrosis factor inducible protein A20; MGC104522; MGC138687; MGC138688; OTU domain containing p

Cat:

SL2803R

Species Reactivity：

Human,Mouse,(predicted: Rat,Dog,Pig,Cow,Horse,)

Immunogen：

KLH conjugated synthetic peptide derived from human TNFAIP3:51-150/790

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TNFAIP3) Polyclonal Antibody, Unconjugated (SL2803R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TNFAIP3) Polyclonal Antibody, Unconjugated (SL2803R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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A20 is a Cys2/Cys2 zinc finger protein that is induced by a variety of inflammatory stimuli and regulates gene expression. Specifically, A20 is induced by tumor necrosis factor (TNF) and interleukin 1 (IL-1), and acts as a negative regulator of nuclear factor κ B (NFκB) gene expression. By inhibiting NFκB activation, A20 plays a critical role in terminating NFκB responses to various stimuli. Although the SLCterminal region of A20 contains seven zinc finger domains, only four of these domains are required for in vitro inhibition of TNF-induced NFκB activation. A20 also interacts with several other proteins, such as TRAF2, TRAF6 and IkB kinase (IKK) γ protein, and can thereby inhibit cell death. TXBP151, a novel A20-binding protein, may mediate the anti-apoptotic activity of A20. Involved in the negative feedback regulation of signal transduction, A20 and A20-binding proteins may be useful as novel therapeutic tools in the treatment of a variety of diseases

Function:
TNFAIP3 interacts with NAF1 and inhibits TNF induced NF kappa B dependent gene expression by interfering with a RIP or TRAF2 mediated transactivation signal. It inhibits programmed cell death, has a role in the function of the lymphoid system, and may contribute to the in vivo effects of TNF.

Subcellular Location:
Cytoplasm. Nucleus.

Similarity:
Belongs to the peptidase C64 family.
Contains 7 A20-type zinc fingers.
Contains 1 OTU domain.

SWISS:
P2316

Gene ID:
7128

Database links:

Entrez Gene: 7128 Human

Entrez Gene: 21929 Mouse

Omim: 191163 Human

SwissProt: P2316 Human

SwissProt: Q60769 Mouse

Unigene: 211600 Human

Unigene: 116683 Mouse

TNFAIP3是一种泛素修饰酶，是机体重要的炎症反应负性调节因子,也是NF-κB的负调节蛋白。TNFAIP3蛋白与很多变态反应疾病的病理生理演变相关联，如：包括类风湿关节炎、冠状动脉疾病、银屑病、腹腔疾病、I型糖尿病、炎症性肠炎和系统性红斑狼疮等。 Picture

Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TNFAIP3) Polyclonal Antibody, Unconjugated (SL2803R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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