KLH conjugated synthetic peptide derived from human MEK6:251-334/334
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Sample: NIH/3T3(Mouse) Cell Lysate at 30 ugPrimary: Anti-MEK6 (SL2911R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 37 kDObserved band size: 35 kDSample: A673(Human) Cell Lysate at 30 ugHela(Human) Cell Lysate at 30 ugPrimary: Anti-MEK6 (SL2911R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 37 kDObserved band size: 35 kD
Mitogen activated protein kinase kinase 6 (MEK6 or MKK6) belongs to the serine/threonine protein kinase family and the MAPK kinase subfamily (MAP2K, MKK or MEKs). MEK6, closely related to MEK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38, thus activating it, in response to inflammatory cytokines and environmental stress.
As an essential component of p38 MAP kinase mediated signal transduction pathway, this protein is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis.
Function: Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively.
Subunit: Interacts with Yersinia yopJ.
Subcellular Location:
Tissue Specificity: Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas.
Post-translational modifications: Weakly autophosphorylated. Phosphorylated by TAOK2. Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
Similarity: Belongs to the enoyl-CoA hydratase/isomerase family.
Sample:
NIH/3T3(Mouse) Cell Lysate at 30 ug
Primary: Anti-MEK6 (SL2911R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 35 kD
Sample:
A673(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-MEK6 (SL2911R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 35 kD
Product Feedback Wall
Specific References (1) | SL2911R has been referenced in 1 publications.
[IF=1.75] Shi, Zeyu, et al. "Effects of Fluoride on the Expression of p38MAPK Signaling Pathway-Related Genes and Proteins in Spleen Lymphocytes of Mice." Biological Trace Element Research (2016): 1-6. WB ; Mouse.
