Rabbit Anti-Phospho-MYL (Ser19) antibody

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Cardiac myosin light chain-2; Cardiac ventricular myosin light chain 2; CMH10; MLC 2v; MLC2; MYL 2; Myosin light chain 2 regulatory cardiac slow; Myosin light polypeptide 2 regulatory cardiac slow; Myosin regulatory light chain 2 ventricular cardiac muscl

Cat:

SL3295R

Species Reactivity：

Mouse,(predicted: Human,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated Synthesised phosphopeptide derived from human MYL2 around the phosphorylation site of Ser19:VF(p-S)MF

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Heart (Rat) Lysate at 40 ugPrimary: Anti-Phospho-MYL(Ser19) (SL3295R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 18 kDObserved band size: 18 kDTissue/cell: smooth muscle of mouse embryo; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Phospho-MYL(Ser19) Polyclonal Antibody, Unconjugated(SL3295R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $256.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]

Subunit:
Myosin is a hexamer of 2 heavy chains and 4 light chains.

Subcellular Location:
Cytoplasm, myofibril, sarcomere, A band

Post-translational modifications:
N-terminus is methylated by METTL11A/NTM1.
Phosphorylated by MYLK3.

DISEASE:
Defects in MYL2 are the cause of familial hypertrophic cardiomyopathy type 10 (CMH10) [MIM:608758]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.

Similarity:
Contains 3 EF-hand domains.

SWISS:
P10916

Gene ID:
4633

Database links:

Entrez Gene: 4633 Human

Entrez Gene: 17906 Mouse

Entrez Gene: 363925 Rat

Omim: 160781 Human

SwissProt: P10916 Human

SwissProt: P51667 Mouse

SwissProt: P08733 Rat

Unigene: 75535 Human

Unigene: 1529 Mouse

Unigene: 37176 Rat

Unigene: 6534 Rat

Picture

Tissue/cell: smooth muscle of mouse embryo; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Phospho-MYL(Ser19) Polyclonal Antibody, Unconjugated(SL3295R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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