Rabbit Anti-ID2 antibody | Sunlong Medical

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GIG-8; GIG8; GIG 8; Helix loop helix protein ID2; ID2A; ID-2; ID2H; Inhibitor of differentiation 2; Inhibitor of DNA binding 2; Inhibitor of DNA binding 2, dominant negative helix loop helix protein; MGC26389; bHLHb26; Cell growth inhibiting gene 8; DNA b

Cat:

SL3515R

Species Reactivity：

Rat,(predicted: Human,Mouse,Chicken,Dog,Pig,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human GIG8/ID2:31-134/134

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ID2) Polyclonal Antibody, Unconjugated (SL3515R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ID2) Polyclonal Antibody, Unconjugated (SL3515R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this gene belongs to the inhibitor of DNA binding (ID) family, and may play a role in negatively regulating cell differentiation. Members of the ID family are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. They inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner, by suppressing their heterodimerization partners through the HLH domains.

Function:
ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression.

Subunit:
Heterodimer with other HLH proteins. Interacts with GATA4, IFI204 and NKX2-5. Interacts with NR0B2.

Subcellular Location:
Cytoplasm. Nucleus.

Tissue Specificity:
Highly expressed in early fetal tissues, including those of the central nervous system.

Similarity:
Contains 1 basic helix-loop-helix (bHLH) domain.

SWISS:
Q02363

Gene ID:
3398

Database links:

Entrez Gene: 3398 Human

Entrez Gene: 15902 Mouse

Omim: 600386 Human

SwissProt: Q02363 Human

SwissProt: P41136 Mouse

Unigene: 36919 Human

Unigene: 34871 Mouse

Picture

Paraformaldehyde-fixed, paraffin embedded (Rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ID2) Polyclonal Antibody, Unconjugated (SL3515R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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