Rabbit Anti-Phospho-Stat2 (Tyr690) antibody

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Stat2 (phospho-Tyr690); Stat2 (phospho Y690); Stat2 (phospho Tyr690); p-Stat2 (Tyr690); signal transducers and activators of transduction2; Homo sapiens interferon alpha induced transcriptional activator; interferon alpha induced transcriptional activator

Cat:

SL3428R

Species Reactivity：

Mouse,(predicted: Human,Rat,Dog,Horse,)

Immunogen：

KLH conjugated Synthesised phosphopeptide derived from human Stat2 around the phosphorylation site of Tyr690:RK(p-Y)LK

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample:Brain (Mouse) Lysate at 40 ugPrimary: Anti-Phospho-Stat2 (Tyr690)(SL3428R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 94kDObserved band size: 105kDParaformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Stat2 (Tyr690)) Polyclonal Antibody, Unconjugated (SL3428R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $256.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. Transcription adaptor P300/CBP (EP300/CREBBP) has been shown to interact specifically with this protein, which is thought to be involved in the process of blocking IFN-alpha response by adenovirus. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010].

Function:
Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.

Subunit:
Interacts with ISGF3G/IRF-9 in the cytoplasm. Heterodimer with STAT1 upon IFN-alpha/beta induced phosphorylation. Interacts with CRSP2 and CRSP6. Interacts with Simian virus 5 protein V and rabies virus phosphoprotein (By similarity). Can form a homodimer upon IFN-alpha induced phosphorylation. Interacts with IFNAR1; the interaction requires the phosphorylation of IFNAR1 at 'Tyr-466'. Interacts with IFNAR2. Interacts with dengue virus NS5; this interaction inhibits the phosphorylation of STAT2, and, when all viral proteins are present (polyprotein), targets STAT2 for degradation. Interacts with human cytomegalovirus/HHSLV5 protein UL123; this interaction promotes viral growth.

Subcellular Location:
Cytoplasm. Nucleus. Note=Translocated into the nucleus upon activation by IFN-alpha/beta.

Post-translational modifications:
Tyrosine phosphorylated in response to IFN-alpha.

Similarity:
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain.

SWISS:
P52630

Gene ID:
6773

Database links:

Entrez Gene: 6773 Human

Entrez Gene: 20847 Mouse

Entrez Gene: 288774 Rat

Omim: 600556 Human

SwissProt: P52630 Human

SwissProt: Q9WVL2 Mouse

Unigene: 530595 Human

Unigene: 293120 Mouse

Unigene: 471333 Mouse

Unigene: 24237 Rat

STAT-信号转导和转录激活因子蛋白家族是近年来发现的一类转录因子.通过JAK-STAT途径介导许多由细胞因子诱导的反应,参与细胞的信号转导和转录。 Picture

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Stat2 (Tyr690)) Polyclonal Antibody, Unconjugated (SL3428R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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