which activates transcription through both consensus and variant cAMP response element (CRE) sites. MECT1 does not appear to modulate CREB1 DNA-binding activity but enhances the interaction of CREB1 with TAF4/TAFII-130. MECT1 translocates with MAML2 (MasterMind-Like Protein 2) to yield a fusion oncogene: t(11;19) (q21;p13). This translocation occurs in mucoepidermoid carcinomas, benign Warthin tumors and clear cell hidradenomas. The novel fusion product that results disrupts the Notch signaling pathway. The fusion protein consists of the N-terminus of MECT1 joined to the SLCterminus of MAML2. The reciprocal fusion protein consisting of the N-terminus of MAML2 joined to the SLCterminus of MECT1 has been detected in a small number of mucoepidermoid carcinomas. Multiple isoforms have been reported for the MECT1 protein.
Subunit:
Binds, as a tetramer, through its N-terminal region, with the bZIP domain of CREB1. 'Arg-314' in the bZIP domain of CREB1 is essential for this interaction. Interaction, via its SLCterminal, with TAF4, enhances recruitment of TAF4 to CREB1. Binds HTLV1 Tax.
Subcellular Location:
Cytoplasm. Nucleus. Note=Cytoplasmic when phosphorylated by SIK or AMPK and when sequestered by 14-3-3 proteins (By similarity). Translocated to the nucleus on Ser-151 dephosphorylation, instigated by a number of factors including calcium ion and cAMP levels.
Tissue Specificity:
Highly expressed in adult and fetal brain. Located to specific regions such as the prefrontal cortex and cerebellum. Very low expression in other tissues such as heart, spleen, lung, skeletal muscle, salivary gland, ovary and kidney.
Post-translational modifications:
Phosphorylation/dephosphorylation states of Ser-151 are required for regulating transduction of CREB activity. TORCs are inactive when phosphorylated, and active when dephosphorylated at this site. This primary site of phosphorylation is mediated by SIKs (SIK1 and SIK2), is regulated by cAMP and calcium levels and is dependent on the phosphorylation of SIKs by LKB1 (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR
DISEASE:
Note=A chromosomal aberration involving CRTC1 is found in mucoepidermoid carcinomas, benign Warthin tumors and clear cell hidradenomas. Translocation t(11;19)(q21;p13) with MAML2. The fusion protein consists of the N-terminus of CRTC1 joined to the SLCterminus of MAML2. The reciprocal fusion protein consisting of the N-terminus of MAML2 joined to the SLCterminus of CRTC1 has been detected in a small number of mucoepidermoid carcinomas.
Similarity:
Belongs to the TORC family.
SWISS:
Q6UUV9
Gene ID:
23373
Database links:
Entrez Gene: 23373?/span>Human
Entrez Gene: 382056?/span>Mouse
Entrez Gene: 684527?/span>Rat
Omim: 607536?/span>Human
SwissProt: Q6UUV9?/span>Human
SwissProt: Q68ED7?/span>Mouse
SwissProt: Q157S1?/span>Rat
Unigene: 371096?/span>Human
Unigene: 428214?/span>Human
Unigene: 227767?/span>Mouse
Unigene: 208248?/span>Rat
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Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Phospho-Torc1(Ser151) Polyclonal Antibody, Unconjugated(SL3453R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Tissue/cell: human brain glioma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Phospho-Torc1(Ser151) Polyclonal Antibody, Unconjugated(SL3453R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Positive control: Hela cells
Concebtration: 5μg/10^6 cells
Incubation conditions: Avoid light , 30 minutes on the ice.
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