Rabbit Anti-Caveolin-3 antibody

Home > Product > Antibody > Rabbit Anti-Caveolin-3 antibody

CAV 3; CAV3; Caveolin3; Caveolin 3; LGMD1C; M caveolin; VIP 21; VIP21; AI385751; Cav-3; caveolin-3; M-cav; M-caveolin; CAV3_MOUSE.

Cat:

SL3610R

Species Reactivity：

Human,(predicted: Mouse,Rat,Dog,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from mouse Caveolin-3:95-151/151

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg /testIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Hela(Human) Cell Lysate at 30 ugPrimary: Anti-Caveolin-3 (SL3610R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 17 kDObserved band size: 17 kDBlank control (blue line):A431(blue). Primary Antibody (green line): Rabbit Anti-Caveolin-3 antibody(SL3610R);Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): F(ab’)2 fragment goat anti-rabbit IgG-FITC;Dilution: 1μg /test. ProtocolThe cells were fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature (The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice.). Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

prev next

Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

Caveolae are specialized domains of the plasma membrane that are implicated in the sequestration of a variety of lipid and protein molecules. It has been suggested that these important cellular organelles have a pivotal role in such diverse biochemical processes as lipid metabolism, growth regulation, signal transduction, and apoptosis. Caveolin interacts with and regulates heterotrimeric G-proteins. Currently, there are three members of the caveolin multigene family which are integral membrane proteins that comprise the major structural component of the caveolar membrane in vivo. Caveolin-2 protein is abundantly expressed in fibroblasts and differentiated adipocytes, smooth and skeletal muscle, and endothelial cells. The expression of caveolin-1 is similar to that of caveolin-2 while caveolin-3 expression appears to be limited to muscle tissue types.

Function:
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. May also regulate voltage-gated potassium channels. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress.

Subunit:
Homooligomer (By similarity). Interacts with DYSF. Interacts with DLG1 and KCNA5; forms a ternary complex. Interacts with DAG1 (via its SLCterminal); the interaction prevents binding of DAG1 with DMD (By similarity). Interacts with TRIM72. Interacts with MUSK; may regulate MUSK signaling.

Subcellular Location:
Golgi apparatus membrane; Peripheral membrane protein (By similarity). Cell membrane; Peripheral membrane protein (By similarity). Membrane, caveola; Peripheral membrane protein (By similarity). Note=Potential hairpin-like structure in the membrane. Membrane protein of caveolae (By similarity).

Tissue Specificity:
Expressed predominantly in muscle.

Post-translational modifications:
Sumoylation with SUMO3 by PIAS4 may reduce agonist-induced internalization and desensitization of adrenergic receptor ABRD2 (By similarity).

Similarity:
Belongs to the caveolin family.

SWISS:
P51637

Gene ID:
12391

Database links:

Entrez Gene: 859 Human

Entrez Gene: 12391 Mouse

Entrez Gene: 29161 Rat

Omim: 601253 Human

SwissProt: P56539 Human

SwissProt: P51637 Mouse

SwissProt: P51638 Rat

Unigene: 98303 Human

Unigene: 3924 Mouse

Unigene: 98191 Rat

Picture

Blank control (blue line):A431(blue).
Primary Antibody (green line): Rabbit Anti-Caveolin-3 antibody(SL3610R); Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): F(ab’)2 fragment goat anti-rabbit IgG-FITC;Dilution: 1μg /test.
Protocol
The cells were fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature (The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice.). Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Product Feedback Wall