The membrane bound adenylyl cyclases (ACs) represent one of the major families of effector enzymes for G protein coupled receptors. Eight human AC isoforms (ASLC1 through ASLC4) have been identified up to now and genes for at least nine adenylate cyclase (ASLC1-ASLC9) have been cloned characterized and sequenced.
This is a calmodulin-sensitive adenylyl cyclase which may be involved in regulatory processes in the central nervous system. It may play a role in neuronal plasticity, memory acquisition and learning and is expressed predominantly in the brain, retina and adrenal medulla.
Function:
This is a calmodulin-sensitive adenylyl cyclase. May be involved in regulatory processes in the central nervous system. It may play a role in memory acquisition and learning.
Subcellular Location:
Membrane; Multi-pass membrane protein.
Tissue Specificity:
Brain, retina and adrenal medulla.
Similarity:
Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
Contains 2 guanylate cyclase domains.
SWISS:
Q08828
Gene ID:
107
Database links:
Entrez Gene: 107 Human
Entrez Gene: 432530 Mouse
Omim: 103072 Human
SwissProt: Q08828 Human
SwissProt: O88444 Mouse
Unigene: 192215 Human
Unigene: 259733 Mouse
腺苷酸环化酶(adenylate cyclase, AC) 是G蛋白偶联受体家族之一，是膜整合蛋白,它的氨基端和羧基端都朝向细胞质。AC在膜的细胞质面有两个催化结构域,还有两个膜整合区,每个膜整合区分别有6个跨膜的α螺旋。哺乳动物中已发现6个腺苷酸环化酶异构体。由于AC能够将ATP转变成cAMP,引起细胞的信号应答,因此,腺苷酸环化酶是G蛋白偶联系统中的效应物。
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Sample:
MCF-7 Cell(Human)Lysate at 30 ug
Primary: Anti- ADCY1 (SL3681R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 123kD
Observed band size: 123kD
Sample:
A549 Cell (Human) Lysate at 30 ug
Primary: Anti- ADCY1 (SL3681R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 123kD
Observed band size: 123kD
Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Adenylate cyclase 1) Polyclonal Antibody, Unconjugated (SL3681R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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