TMEM16A (Transmembrane protein 16A) is an eight-transmembrane protein homologous to C12orf3, C11orf25 and FLJ34272 gene products and is amplified in various cancers. It acts as a calcium-activated chloride channel and is required for normal tracheal development.
Function:
Acts as a calcium-activated chloride channel. Required for normal tracheal development (By similarity).
Subcellular Location:
Cell membrane; Multi-pass membrane protein. Cytoplasm.
Tissue Specificity:
Broadly expressed with higher levels in liver and skeletal muscle.
Similarity:
Belongs to the anoctamin family.
SWISS:
Q5XXA6
Gene ID:
55107
Database links:
Entrez Gene: 55107 Human
Entrez Gene: 101772 Mouse
Entrez Gene: 309135 Rat
Omim: 610108 Human
SwissProt: Q5XXA6 Human
SwissProt: Q8SHY3 Mouse
Unigene: 503074 Human
Unigene: 26700 Mouse
Unigene: 162509 Rat
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Sample:
Lane 1: Skeletal muscle (Mouse) Lysate at 40 ug
Lane 2: Skeletal muscle (Rat) Lysate at 40 ug
Lane 3: HepG2 (Human) Cell Lysate at 30 ug
Lane 4: A673 (Human) Cell Lysate at 30 ug
Lane 5: U-2OS (Human) Cell Lysate at 30 ug
Lane 6: MKN45 (Human) Cell Lysate at 30 ug
Primary: Anti-ANO1/TMEM16A (SL3794R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 114-120 kD
Observed band size: 120 kD
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-TMEM16A Polyclonal Antibody, Unconjugated(SL3794R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Tissue/cell: Human lung cancer tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-TMEM16A Polyclonal Antibody, Unconjugated(SL3794R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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