Rabbit Anti-ANO1 antibody | Sunlong Medical

Home > Product > Antibody > Rabbit Anti-ANO1 antibody

ANO 1; ANO1_HUMAN; Anoctamin 1; Anoctamin 1 calcium activated chloride channel; Anoctamin-1; Anoctamin1; Discovered on gastrointestinal stromal tumors protein 1; DOG 1; FLJ10261; Membrane protein; Oral cancer overexpressed 2; Oral cancer overexpressed pro

Cat:

SL3794R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Pig,Cow,Horse,)

Immunogen：

KLH conjugated synthetic peptide derived from human DOG-1/TMEM16A:151-250/986

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Skeletal muscle (Mouse) Lysate at 40 ugLane 2: Skeletal muscle (Rat) Lysate at 40 ugLane 3: HepG2 (Human) Cell Lysate at 30 ugLane 4: A673 (Human) Cell Lysate at 30 ugLane 5: U-2OS (Human) Cell Lysate at 30 ugLane 6: MKN45 (Human) Cell Lysate at 30 ugPrimary: Anti-ANO1/TMEM16A (SL3794R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 114-120 kDObserved band size: 120 kDTissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-TMEM16A Polyclonal Antibody, Unconjugated(SL3794R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: Human lung cancer tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-TMEM16A Polyclonal Antibody, Unconjugated(SL3794R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

prev next

Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

TMEM16A (Transmembrane protein 16A) is an eight-transmembrane protein homologous to C12orf3, C11orf25 and FLJ34272 gene products and is amplified in various cancers. It acts as a calcium-activated chloride channel and is required for normal tracheal development.

Function:
Acts as a calcium-activated chloride channel. Required for normal tracheal development (By similarity).

Subcellular Location:
Cell membrane; Multi-pass membrane protein. Cytoplasm.

Tissue Specificity:
Broadly expressed with higher levels in liver and skeletal muscle.

Similarity:
Belongs to the anoctamin family.

SWISS:
Q5XXA6

Gene ID:
55107

Database links:

Entrez Gene: 55107 Human

Entrez Gene: 101772 Mouse

Entrez Gene: 309135 Rat

Omim: 610108 Human

SwissProt: Q5XXA6 Human

SwissProt: Q8SHY3 Mouse

Unigene: 503074 Human

Unigene: 26700 Mouse

Unigene: 162509 Rat

Picture

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-TMEM16A Polyclonal Antibody, Unconjugated(SL3794R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: Human lung cancer tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-TMEM16A Polyclonal Antibody, Unconjugated(SL3794R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Product Feedback Wall