Rabbit Anti-GDF15 antibody | Sunlong Medical

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GDF 15; GDF-15; GDF15_HUMAN; Growth differentiation factor 15; Growth/differentiation factor 15; Macrophage inhibitory cytokine 1; MIC 1; Mic-1; MIC1; NAG 1; NAG-1; NAG1; NRG 1; NRG-1; NRG1; NSAID (nonsteroidal anti inflammatory drug) acti

Cat:

SL3818R

Species Reactivity：

Human,Mouse,Rat,

Immunogen：

KLH conjugated synthetic peptide derived from human GDF-15:221-308/308

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Urinary bladder (Mouse) Lysate at 40 ug Lane 2: Large intestine (Mouse) Lysate at 40 ug Lane 3: Urinary bladder (Rat) Lysate at 40 ug Lane 4: Large intestine (Rat) Lysate at 40 ug Lane 5: HT1080 (Human) cell Lysate at 40 ug Lane 6: HepG2 (Human) cell Lysate at 40 ug Lane 7: K562 (Human) cell Lysate at 40 ug Lane 8: A549 (Human) cell Lysate at 40 ug Primary: Anti-GDF15 (SL3818R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 35 kDSample: K562 Cell Lysate at 30 ugA431 Cell Lysate at 30 ugPrimary: Anti-GDF15 (SL3818R) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(SL0295G-HRP) at 1: 5000 dilution; Predicted band size:34 kDObserved band size:30 kDParaformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (GDF15) Polyclonal Antibody, Unconjugated (SL3818R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Growth differentiation factor 15 (GDF15) is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. It has been implicated in a variety of functions directly related to tumorigenicity including antiproliferative and pro-apoptotic effects. BMP proteins are secreted growth factors that are characterized by seven conserved cysteine residues. In general, they are regulators of cell growth and differentiation in both embryonic and adult tissues. GDF15 is an important downstream mediator of DNA damage signaling and a transcriptional target of p53.

Subcellular Location:
Secreted.

Tissue Specificity:
Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney.

Similarity:
Belongs to the TGF-beta family.

SWISS:
Q99988

Gene ID:
9518

Database links:

Entrez Gene: 9518 Human

Entrez Gene: 23886 Mouse

Entrez Gene: 29455 Rat

Omim: 605312 Human

SwissProt: Q99988 Human

SwissProt: Q9Z0J7 Mouse

SwissProt: Q9Z0J6 Rat

Unigene: 616962 Human

Unigene: 31325 Mouse

Unigene: 44228 Rat

生长分化因子15（GDF15）是骨形态发生蛋白（BMP）和转化生长因子-β超家族成员。GDF-15又叫巨噬细胞抑制因子（macrophage inhibiting cytokine，MIC）-1、胎盘转化生长因子（placental transformation growth factor，PTGF）-β、前列腺衍生因子（prostate derived factor，PDF）和胎盘骨形态发生蛋白（placental bone morphogeneticprotein，PLAB）。
有学者认为：GDF-15在肿瘤发展的不同阶段发挥着不同的生物学作用；随着肿瘤的发生发展，GDF-15可能表达不同形式的蛋白，在肿瘤发生的早期阶段可能发挥抑瘤作用，而在肿瘤发展的后期阶段发挥促瘤作用。 Picture

Sample:
K562 Cell Lysate at 30 ug
A431 Cell Lysate at 30 ug
Primary: Anti-GDF15 (SL3818R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(SL0295G-HRP) at 1: 5000 dilution;
Predicted band size:34 kD
Observed band size:30 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (GDF15) Polyclonal Antibody, Unconjugated (SL3818R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

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