Rabbit Anti-TNFSF15 antibody

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TL1A; TL1 A; TL 1A; TL-1A; tl1; tl1a; tnf ligand related molecule 1; TNF ligand-related molecule 1; tnf superfamily ligand tl1a; TNF15_HUMAN; Tumor necrosis factor ligand superfamily member 15; Vascular endothelial cell growth inhibitor; Vascular endothel

Cat:

SL5092R

Species Reactivity：

Human,(predicted: Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,Guinea Pig,)

Immunogen：

KLH conjugated synthetic peptide derived from human TNFSF15:161-251/251<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample:bone(mouse) Lysate at 40 ugPrimary: Anti-TNFSF15 (SL5092R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 28kDObserved band size: 38 kDTissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-TNFSF15 Polyclonal Antibody, Unconjugated(SL5092R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Receptor for TNFRSF25 and TNFRSF6B. Mediates activation of NF-kappa-B. Inhibits vascular endothelial growth and angiogenesis (in vitro). Promotes activation of caspases and apoptosis.

Function:
Receptor for TNFRSF25 and TNFRSF6B. Mediates activation of NF-kappa-B. Inhibits vascular endothelial growth and angiogenesis (in vitro). Promotes activation of caspases and apoptosis.

Subunit:
Homotrimer.

Subcellular Location:
Membrane; Single-pass type II membrane protein (Probable). Tumor necrosis factor ligand superfamily member 15, secreted form: Secreted.

Tissue Specificity:
Specifically expressed in endothelial cells. Detected in monocytes, placenta, lung, liver, kidney, skeletal muscle, pancreas, spleen, prostate, small intestine and colon.

Similarity:
Belongs to the tumor necrosis factor family.

SWISS:
O95150

Gene ID:
9966

Database links:

Entrez Gene: 9966 Human

Entrez Gene: 326623 Mouse

Entrez Gene: 252878 Rat

Omim: 604052 Human

SwissProt: O95150 Human

SwissProt: Q5UBV8 Mouse

SwissProt: Q8K3Y7 Rat

Unigene: 241382 Human

Unigene: 208152 Mouse

Unigene: 84873 Rat

Picture

Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-TNFSF15 Polyclonal Antibody, Unconjugated(SL5092R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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