Rabbit Anti-PDK1/Mitochondrial Pyruvate dehydrogenase kinase 1 antibody

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PDHK1; mitochondrial; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1; HGNC:8809; Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1; PDK 1; PDK-1; Pyruvate dehydrogenase kinase isoform 1; Pyruvate dehydrogenase kinase, isoenzyme 1; PDK1_HUMAN.

Cat:

SL3788R

Species Reactivity：

Mouse,(predicted: Human,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human PDK1:51-150/436

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Heart (Mouse) Lysate at 40 ugPrimary: Anti- PDK1/Mitochondrial Pyruvate dehydrogenase kinase 1(SL3788R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 45 kDObserved band size: 50 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PDK1/Mitochondrial Pyruvate dehydrogenase kinase 1) Polyclonal Antibody, Unconjugated (SL3788R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2013]

Function:
Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

Subcellular Location:
Mitochondrion matrix.

Tissue Specificity:
Expressed predominantly in the heart.

Similarity:
Belongs to the PDK/BCKDK protein kinase family.
Contains 1 histidine kinase domain.

SWISS:
Q15118

Gene ID:
5163

Database links:

Entrez Gene: 5163 Human

Entrez Gene: 228026 Mouse

Entrez Gene: 116551 Rat

Omim: 602524 Human

SwissProt: Q15118 Human

SwissProt: Q8SFP9 Mouse

SwissProt: Q63065 Rat

Unigene: 470633 Human

Unigene: 34411 Mouse

Unigene: 11185 Rat

Picture

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PDK1/Mitochondrial Pyruvate dehydrogenase kinase 1) Polyclonal Antibody, Unconjugated (SL3788R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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