Rabbit Anti-NCF1 antibody | Sunlong Medical

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47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NADPH oxidase organizer 2; NCF 47K; NCF-1; NCF-47K; Ncf1; NCF1_HUMAN; Neutrophil cytosol factor 1; Neutrophil cytosolic factor 1; Neutrophil NADPH oxidase factor 1;

Cat:

SL3886R

Species Reactivity：

Human,Mouse,(predicted: Rat,Dog,)

Immunogen：

KLH conjugated synthetic peptide derived from human NCF1:151-250/390

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000Flow-Cyt=1ug/testnot yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Bone (Mouse) Lysate at 40 ugLymph node (Mouse) Lysate at 40 ugSpleen (Mouse) Lysate at 40 ugPrimary: Anti-NCF1 (SL3886R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 45 kDObserved band size: 48 kDSample: Lane 1: Lung (Mouse) Lysate at 40 ugLane 2: Spleen (Mouse) Lysate at 40 ugLane 3: Lymph node (Mouse) Lysate at 40 ugPrimary: Anti-NCF1 (SL3886R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 48 kDObserved band size: 48 kDU-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with NCF1 Antibody(SL3886R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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NCF1, along with NCF2 and a membrane bound cytochrome b558, is required for activation of the latent NADPH oxidase necessary for superoxide production. Defects in NCF1 are the cause of autosomal cytochrome-b-positive chronic granulomatous disease type 1 (CGD).

Function:
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

Subunit:
Interacts with NOXA1. Interacts with ADAM15. Interacts with TRAF4. Interacts with FASLG.

Subcellular Location:
Cytoplasm.

Post-translational modifications:
Phosphorylated by PRKCD; phosphorylation induces activation of NCF1 and NADPH oxidase activity.

DISEASE:
Granulomatous disease, chronic, cytochrome-b-positive 1, autosomal recessive (CGD1) [MIM:233700]: A disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity:
Contains 1 PX (phox homology) domain.
Contains 2 SH3 domains.

SWISS:
P14598

Gene ID:
653361

Database links:

Entrez Gene: 281345 Cow

Entrez Gene: 653361 Human

Entrez Gene: 17969 Mouse

Entrez Gene: 100134857 Pig

Entrez Gene: 100001763 Rabbit

Entrez Gene: 114553 Rat

Omim: 608512 Human

SwissProt: O77774 Cow

SwissProt: P14598 Human

SwissProt: Q09014 Mouse

Unigene: 647047 Human

Unigene: 655201 Human

Unigene: 425296 Mouse

Unigene: 38575 Rat

Picture

Sample:
Lane 1: Lung (Mouse) Lysate at 40 ug
Lane 2: Spleen (Mouse) Lysate at 40 ug
Lane 3: Lymph node (Mouse) Lysate at 40 ug
Primary: Anti-NCF1 (SL3886R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD

U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with NCF1 Antibody(SL3886R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

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