Home > Product > Antibody > Rabbit Anti-GTPBP10 antibody
BC034507; FLJ38242; GTP binding protein 10 (putative); GTP-binding protein 10; GTPBA_HUMAN; GTPBP10; MGC104191; MGC30495; ObgH2; Protein obg homolog 2.
Cat:
SL3893R
Species Reactivity:
Human,Mouse,Rat,(predicted: Dog,Pig,Cow,Horse,Rabbit,)
Immunogen:
KLH conjugated synthetic peptide derived from human GTPBP10:151-250/387
Format:
Liquid
Storage instructions:
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration:
1mg/ml
Clonality:
Polyclonal
Isotype:
IgG
Applications:
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500(Paraffin sections need to do antigen repair)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host:
Rabbit
Product Overview:
Sample: Skin (Mouse) Lysate at 40 ugPrimary: Anti-GTPBP10 (SL3893R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 43 kDObserved band size: 45 kDTissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-GTPBP10 Polyclonal Antibody, Unconjugated(SL3893R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
More
Unit:
Price: $
Product PDFs
Datasheet:


GTP-binding protein 10 (GTPBP10) belongs to the GTP1/OBG family. Small G proteins, such as GTPBP10, act as molecular switches that play crucial roles in the regulation of fundamental cellular processes such as protein synthesis, nuclear transport, membrane trafficking, and signal transduction; GTPBA_HUMAN; GTP-binding protein 10; Protein obg homolog 2; ObgH2.

Function:
May be involved in the ribosome maturation process. Complements an ObgE(CgtA) function in E.coli ribosome maturation. Plays a role of GTPase in vitro. When missing, disorganization of the nuceleolar architecture is observed.

Subcellular Location:
Nucleus, nucleolus. Chromosome. Note=Found in the dense fibrillar compartment region of the nucleolus. At the onset of mitosis moves to the chromosome surface and remains there until anaphase. Gradually re-assembles into the nucleolus at late anaphase to telophase.

Similarity:
Belongs to the GTP1/OBG family.

SWISS:
A4D1E9

Gene ID:
85865

Database links:

Entrez Gene: 85865 Human

Entrez Gene: 207704 Mouse

Omim: 610920 Human

SwissProt: A4D1E9 Human

SwissProt: Q8K013 Mouse

Unigene: 593547 Human

Unigene: 709271 Human

Unigene: 323984 Mouse



Picture

Sample:
Skin (Mouse) Lysate at 40 ug
Primary: Anti-GTPBP10 (SL3893R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 45 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-GTPBP10 Polyclonal Antibody, Unconjugated(SL3893R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Product Feedback Wall
Message :
Your Email :
Copyright © 2007-2018 Sunlong Medical All Rights Reserved.