Sample:
A431(Human) Lysate at 30 ug
U251(Human) Lysate at 30 ug
Huvec(Human) Lysate at 30 ug
Raji(Human) Lysate at 30 ug
U937(Human) Lysate at 30 ug
Primary: Anti- phospho-SLCMyc(Thr358) (SL4017R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 62 kD
Blank control (blue line): Hela cells (fixed with 70% methanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃).
Primary Antibody (green line): Rabbit Anti-phospho-SLCMyc(Thr358) antibody (SL4017R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.