Rabbit Anti-GKRP antibody | Sunlong Medical

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FGQTL5; GCK; GCKR; GCKR_HUMAN; GKRP; GLRE; glucokinase (hexokinase 4) regulator; Glucokinase; Glucokinase regulator; Glucokinase regulatory protein; Hexokinase type IV; Hexokinase-4; Hexokinase-D; HK IV; HK4; OTTHUMP00000158533.

Cat:

SL2356R

Species Reactivity：

Human,Mouse,(predicted: Rat,Dog,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human GKRP:261-360/625

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Liver (Mouse) Lysate at 40 ugA549(Human) Cell Lysate at 30 ug U87MG(Human) Cell Lysate at 30 ugHepG2(Human) Cell Lysate at 30 ugPrimary: Anti- GKRP (SL2356R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 69 kDObserved band size: 69 kDParaformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GKRP) Polyclonal Antibody, Unconjugated (SL2356R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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GCKR is a subfamily member of the SIS (Sugar ISomerase) family of proteins. It is a regulatory protein that inhibits glucokinase in liver and pancreatic islet cells by binding non-covalently to form an inactive complex with the enzyme. This gene is considered a susceptibility gene candidate for a form of maturity-onset diabetes of the young.

Function:
Inhibits glucokinase by forming an inactive complex with this enzyme.

Tissue Specificity:
Found in liver and pancreas. Not detected in muscle, brain, heart, thymus, intestine, uterus, adipose tissue, kidney, adrenal, lung or spleen.

Similarity:
Belongs to the GCKR family.
Contains 2 SIS domains.

SWISS:
Q14397

Gene ID:
2646

Database links:
UniProtKB/Swiss-Prot: Q14397.6

Picture

Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GKRP) Polyclonal Antibody, Unconjugated (SL2356R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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