Rabbit Anti-SOAT2 antibody | Sunlong Medical

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Acat2; acetoacetyl CoA thiolase; acetoacetyl Coenzyme A thiolase; Acetyl CoA acetyltransferase; Acetyl CoA transferase like protein; acetyl Coenzyme A acetyltransferase 2; Acetyl-CoA acetyltransferase; Acetyl-CoA transferase-like protein; Acyl Coenzyme A

Cat:

SL5020R

Species Reactivity：

Human,Rat,(predicted: Mouse,Dog,Pig,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human ACAT2:331-430/522

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: K562(Human) Cell Lysate at 30 ugJurkat(Human) Cell Lysate at 30 ugPrimary: Anti-SOAT2 (SL5020R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 40 kDObserved band size: 40 kD Paraformaldehyde-fixed, paraffin embedded (Rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SOAT2) Polyclonal Antibody, Unconjugated (SL5020R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Acetyl-Coenzyme A acetyltransferase 2 is an enzyme involved in lipid metabolism. Reported patients with ACAT2 deficiency have shown severe mental retardation and hypotonus. The ACAT2 gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation.

Function:
Plays a role in lipoprotein assembly and dietary cholesterol absorption. In addition to its acyltransferase activity, it may act as a ligase. May provide cholesteryl esters for lipoprotein secretion from hepatocytes and intestinal mucosa.

Subunit:
May form homo- or heterodimers (By similarity).

Subcellular Location:
Endoplasmic reticulum membrane; Multi-pass membrane protein.

Similarity:
Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.

SWISS:
O75908

Gene ID:
8435

Database links:

Entrez Gene: 8435 Human

Omim: 601311 Human

SwissProt: O75908 Human

Unigene: 20580 Human

Picture

Paraformaldehyde-fixed, paraffin embedded (Rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SOAT2) Polyclonal Antibody, Unconjugated (SL5020R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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