Rabbit Anti-AGPAT1 antibody

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1 AGPAT1; LPAAT alpha; LPAATA; MGC5423; 1 acyl sn glycerol 3 phosphate acyltransferase alpha; 1 acylglycerol 3 phosphate O acyltransferase 1 (acetoacetly Coenzyme A thiolase) ; 1 acylglycerol 3 phosphate O acyltransferase 1 (lysophosphatidic acid acyltran

Cat:

SL5023R

Species Reactivity：

Mouse,Rat,(predicted: Human,Dog,Pig,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human AGPAT1:101-200/283

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Siver (Mouse) Lysate at 40 ugPrimary: Anti-AGPAT1 (SL5023R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 31 kDObserved band size: 31 kDParaformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AGPAT1) Polyclonal Antibody, Unconjugated (SL5023R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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AGPAT1 converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone.

Function:
Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone.

Subcellular Location:
Membrane; Multi-pass membrane protein

Similarity:
Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.

SWISS:
Q99943

Gene ID:
10554

Database links:

Entrez Gene: 10554 Human

Omim: 603099 Human

SwissProt: Q99943 Human

Unigene: 409230 Human

Picture

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AGPAT1) Polyclonal Antibody, Unconjugated (SL5023R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

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