Rabbit Anti-ME1 antibody | Sunlong Medical

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Cytosolic malic enzyme 1; HUMNDME; Malate dehydrogenase; Malic enzyme 1; Malic enzyme 1, NADP(+) dependent, cytosolic; Malic enzyme 1, soluble; Malic enzyme, cytoplasmic; MES; NADP dependent malic enzyme; NADP ME; Pyruvic malic carboxylase.Cytosolic malic

Cat:

SL5084R

Species Reactivity：

Rat,(predicted: Human,Mouse,Dog,Pig,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human NADP ME:451-550/572

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Hela(human)cell Lysate at 30 ugPrimary: Anti- ME1 (SL5084R)at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 64kDObserved band size: 64kD Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ME1) Polyclonal Antibody, Unconjugated (SL5084R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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ME1 is a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Expressed in all tissues tested including liver, placenta and white adipose tissue.

Similarity:
Belongs to the malic enzymes family.

SWISS:
P48163

Gene ID:
4199

Database links:

Entrez Gene: 4199 Human

Entrez Gene: 17436 Mouse

Entrez Gene: 24552 Rat

Omim: 154250 Human

SwissProt: P48163 Human

SwissProt: P01361 Mouse

SwissProt: P13697 Rat

Unigene: 21160 Human

Unigene: 148155 Mouse

Unigene: 3519 Rat

Picture

Sample:
Hela(human)cell Lysate at 30 ug
Primary: Anti- ME1 (SL5084R)at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64kD
Observed band size: 64kD

Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ME1) Polyclonal Antibody, Unconjugated (SL5084R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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