The enzyme mevalonate pyrophosphate decarboxylase (MVD) catalyzes the conversion of mevalonate pyrophosphate into isopentenyl pyrophosphate in one of the early steps in cholesterol biosynthesis. It decarboxylates and dehydrates its substrate while hydrolyzing ATP.
Function:
Performs the first committed step in the biosynthesis of isoprenes.
Subunit:
Homodimer.
Tissue Specificity:
Expressed in heart, skeletal muscle, lung,liver, brain, pancreas, kidney and placenta.
Similarity:
Belongs to the diphosphomevalonate decarboxylase family.
SWISS:
P5722
Gene ID:
4597
Database links:
Entrez Gene: 4597 Human
Entrez Gene: 192156 Mouse
Entrez Gene: 81726 Rat
Omim: 603236 Human
SwissProt: Q0P570 Cow
SwissProt: P5722 Human
SwissProt: Q99JF5 Mouse
SwissProt: Q62967 Rat
Unigene: 252457 Human
Unigene: 28146 Mouse
Unigene: 10288 Rat
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Sample:
H9C2(Rat) Cell Lysate at 30 ug
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti-MVD (SL5085R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 43 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MVD) Polyclonal Antibody, Unconjugated (SL5085R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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