Rabbit Anti-HLA-A antibody | Sunlong Medical

Home > Product > Antibody > Rabbit Anti-HLA-A antibody

Major Histocompatibility Complex, Class I; HLA Class I Histocompatibility Antigen, A Alpha Chain; HLAA; HLA Class I Histocompatibility Antigen, A-1 Alpha Chain; MHC Class I Antigen HLA-A Heavy Chain; Leukocyte Antigen Class I-A; Human Leukocyte Antigen A;

Cat:

SL2387R

Species Reactivity：

Mouse,Rat,(predicted: Human,)

Immunogen：

KLH conjugated synthetic peptide derived from human HLA-A:51-150/365

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

IHC-P=1:100-500IHC-F=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Tissue/cell: mouse lymphoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-MHC class I Polyclonal Antibody, Unconjugated(SL2387R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingThe blue histogram is unstained cells(RSC96 cells). The Wathet Blue histogram is cells stained with secondary antibody alone. The Orange histogram is cells stained with rabbit IgG isotype control antibody plus secondary antibody. The green histogram is cells stained with Rabbit Anti-MHC class I/HLA A antibody (SL2387R)plus secondary antibody.Concebtration:5μg/10^6 cells .

prev next

Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen so that they can be recognized by cytotoxic T cells. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. More than 6000 HLA-A alleles have been described. The HLA system plays an important role in the occurrence and outcome of infectious diseases, including those caused by the malaria parasite, the human immunodeficiency virus (HIV), and the severe acute respiratory syndrome coronavirus (SARS-CoV). The structural spike and the nucleocapsid proteins of the novel coronavirus SARS-CoSLV2, which causes coronavirus disease 2019 (COVID-19), are reported to contain multiple Class I epitopes with predicted HLA restrictions. Individual HLA genetic variation may help explain different immune responses to a virus across a population.[provided by RefSeq, Aug 2020]

Function:
HLA-A (Major Histocompatibility Complex, Class I, A) is a Protein Coding gene. Diseases associated with HLA-A include Severe Cutaneous Adverse Reaction and Birdshot Chorioretinopathy. Among its related pathways are HIV Life Cycle and Nef-mediates down modulation of cell surface receptors by recruiting them to clathrin adapters. Gene Ontology (GO) annotations related to this gene include RNA binding and peptide antigen binding. An important paralog of this gene is HLA-B.

Subunit:
Heterodimer of an alpha chain and a beta chain (beta-2-microglobulin). Interacts with human herpesvirus 8 MIR1 protein. Interacts with HTLSLV1 p12I accessory protein.

Subcellular Location:
Cell Membrane; Type I membrane protein.

Tissue Specificity:
Ubiquitous.

Post-translational modifications:
Polyubiquitinated in a post ER compartment by interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system.

Similarity:
Belongs to the MHC class I family.
highly similar to HLA class I histocompatibility antigen, A-3 alpha chain

SWISS:
P04439

Gene ID:
3105

Database links:

Entrez Gene: 3105 Human

Omim: 142800 Human

SwissProt: P04439 Human

Picture

The blue histogram is unstained cells(RSC96 cells).
The Wathet Blue histogram is cells stained with secondary antibody alone.
The Orange histogram is cells stained with rabbit IgG isotype control antibody plus secondary antibody.
The green histogram is cells stained with Rabbit Anti-MHC class I/HLA A antibody (SL2387R)plus secondary antibody. Concebtration:5μg/10^6 cells .

Product Feedback Wall