Rabbit Anti-phospho-CREM (Ser271 + Ser274) antibody

Home > Product > Antibody > Rabbit Anti-phospho-CREM (Ser271 + Ser274) antibody

CREM (phospho S271 + S274); p-CREM (phospho S271 + S274); cAMP response element modulator; cAMP responsive element modulator; hCREM 2; hCREM2; ICER; Inducible cAMP early repressor; Inducible cAMP early repressor ICER; MGC111110; MGC17881; MGC41893.

Cat:

SL4066R

Species Reactivity：

Mouse,Rat,(predicted: Human,Chicken,Pig,Horse,)

Immunogen：

KLH conjugated Synthesised phosphopeptide derived from human CREM around the phosphorylation site of Ser271 + Ser274:AA(p-S)PG(p-S)LH

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-CREM (Ser271 +Ser 274)) Polyclonal Antibody, Unconjugated (SL4066R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-CREM (Ser271 +Ser 274)) Polyclonal Antibody, Unconjugated (SL4066R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

prev next

Unit:

Price: $256.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

CREM is a a bZIP transcription factor that binds to the cAMP responsive element found in many viral and cellular promoters. It is derived from a multiexonic gene that encodes both activators and antagonists of cAMP-inducible transcription by differential splicing. Splice variants with antagonistic function lack 2 glutamine-rich domains and block cAMP-induced transcription, whereas an isoform that includes these glutamine-rich domains is a transcriptional activator.

Function:
Transcriptional regulator that binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. Isoforms are either transcriptional activators or repressors. Plays a role in spermatogenesis and is involved in spermatid maturation.

Subunit:
Binds DNA as a dimer. Interacts with FHL5. Interacts with CDC34.

Subcellular Location:
Nucleus.

Tissue Specificity:
Expressed in testes (round spermatids) (at protein level). Isoform 14 is the major activator form in testes.

Post-translational modifications:
Isoform 9 is ubiquitinated by CDC34 and RAD6B in order to be degraded by the proteasome.
Stimulated by phosphorylation.

Similarity:
Belongs to the bZIP family.
Contains 1 bZIP domain.
Contains 1 KID (kinase-inducible) domain.

SWISS:
Q03060

Gene ID:
1390

Database links:

Entrez Gene: 403887 Dog

Entrez Gene: 1390 Human

Entrez Gene: 12916 Mouse

Entrez Gene: 25620 Rat

Omim: 123812 Human

SwissProt: P79145 Dog

SwissProt: Q03060 Human

SwissProt: P27699 Mouse

SwissProt: Q03061 Rat

Unigene: 200250 Human

Unigene: 10251 Rat

Picture

Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-CREM (Ser271 +Ser 274)) Polyclonal Antibody, Unconjugated (SL4066R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Product Feedback Wall