Rabbit Anti-Squalene Epoxidase antibody

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ERG1; SE antibody SQLE; Squalene monooxygenase; ERG1_HUMAN.

Cat:

SL4218R

Species Reactivity：

Human,Rat,(predicted: Mouse,Chicken,Dog,Pig,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human Squalene Epoxidase:101-200/574

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: NIH/3T3(Mouse) Cell Lysate at 30 ugA549(Human) Cell Lysate at 30 ugPrimary: Anti-Squalene Epoxidase (SL4218R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 64 kDObserved band size: 64 kDTissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Squalene Epoxidase Polyclonal Antibody, Unconjugated(SL4218R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This protein catalyzes the first oxygenation step in sterol biosynthesis and is suggested to be one of the rate-limiting enzymes in this pathway.

Function:
Catalyzes the first oxygenation step in sterol biosynthesis and is suggested to be one of the rate-limiting enzymes in this pathway.

Subunit:
May form a complex with squalene synthase.

Subcellular Location:
Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.

Similarity:
Belongs to the squalene monooxygenase family.

SWISS:
Q14534

Gene ID:
6713

Database links:

Entrez Gene: 6713 Human

Entrez Gene: 20775 Mouse

Entrez Gene: 29230 Rat

Omim: 602019 Human

SwissProt: Q14534 Human

SwissProt: P52019 Mouse

SwissProt: P52020 Rat

Unigene: 71465 Human

Unigene: 296169 Mouse

Unigene: 33239 Rat

Picture

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Squalene Epoxidase Polyclonal Antibody, Unconjugated(SL4218R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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