Rabbit Anti-VLDL Receptor antibody

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Very low density lipoprotein receptor; VLDL R; VLDLR; VLDL-R; VLDLRCH.

Cat:

SL4228R

Species Reactivity：

Human,

Immunogen：

KLH conjugated synthetic peptide derived from human VLDL Receptor:551-650/873<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ICC=1:100not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (VLDL Receptor) polyclonal Antibody, Unconjugated (SL4228R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.Blank control: 293T(blue). Primary Antibody:Rabbit Anti-VLDL Receptor antibody(SL4228R), Dilution: 5μg in 100 1μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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VLDL (very low density lipoprotein) cholesterol is one of the three major types of cholesterol found in blood. VLDL receptor plays an essential role in triglyceride metabolism. It binds VLDL and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin coated pits. Binding to Reelin induces tyrosine phosphorylation of Dab1 and modulation of Tau phosphorylation.

Function:
Binds VLDL and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. Binding to Reelin induces tyrosine phosphorylation of Dab1 and modulation of Tau phosphorylation (By similarity).

Subunit:
Binds to the extracellular matrix protein Reelin. Interacts with VLDLR. Interacts with SNX17. Interacts with DAB1. Receptor for the minor-group human rhinoviruses (HRVs); binds protein VP1 through the second and third LDL-receptor class A domains.

Subcellular Location:
Membrane; Single-pass type I membrane protein. Membrane, clathrin-coated pit; Single-pass type I membrane protein.

Tissue Specificity:
Abundant in heart and skeletal muscle; also ovary and kidney; not in liver.

Post-translational modifications:
Ubiquitinated at Lys-839 by MYLIP leading to degradation.

DISEASE:
Defects in VLDLR are the cause of cerebellar ataxia mental retardation and dysequilibrium syndrome type 1 (CMARQ1) [MIM:24850]; also known as dysequilibrium syndrome (DES) or non-progressive cerebellar disorder with mental retardation. CMARQ1 is a congenital, non-progressive cerebellar ataxia associated with disturbed equilibrium, delayed ambulation, mental retardation and cerebellar hypoplasia. Additional features include short stature, strabismus, pes planus and, rarely, seizures.

Similarity:
Contains 3 EGF-like domains.
Contains 8 LDL-receptor class A domains.
Contains 6 LDL-receptor class B repeats.

SWISS:
P98155

Gene ID:
7436

Database links:

Entrez Gene: 7436 Human

Entrez Gene: 22359 Mouse

Entrez Gene: 25696 Rat

Omim: 192977 Human

SwissProt: P98155 Human

SwissProt: P98156 Mouse

SwissProt: P98166 Rat

Unigene: 370422 Human

Unigene: 4141 Mouse

Unigene: 9975 Rat

Picture

Blank control: 293T(blue).
Primary Antibody:Rabbit Anti-VLDL Receptor antibody(SL4228R), Dilution: 5μg in 100 1μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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