Rabbit Anti-USP28 antibody | Sunlong Medical

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Deubiquitinating enzyme 28; KIAA1515; Ubiquitin carboxyl terminal hydrolase 28; Ubiquitin carboxyl-terminal hydrolase 28; Ubiquitin specific peptidase 28; Ubiquitin specific processing protease 28; Ubiquitin specific protease 28; Ubiquitin thioesterase 28

Cat:

SL5820R

Species Reactivity：

Mouse,(predicted: Human,Rat,)

Immunogen：

KLH conjugated synthetic peptide derived from human USP28:101-200/1077

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Heart (Mouse) Lysate at 40 ugPrimary: Anti-Hairless (SL5820R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 127 kDObserved band size: 127 kDParaformaldehyde-fixed, paraffin embedded (mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (USP28) Polyclonal Antibody, Unconjugated (SL5820R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus.

SWISS:
Q96RU2

Gene ID:
57646

Database links:

Entrez Gene: 57646 Human

Entrez Gene: 235323 Mouse

Entrez Gene: 315639 Rat

Omim: 610748 Human

SwissProt: Q96RU2 Human

SwissProt: Q5I043 Mouse

SwissProt: D3ZJ96 Rat

Unigene: 503891 Human

Unigene: 21630 Mouse

Unigene: 6599 Rat

Picture

Paraformaldehyde-fixed, paraffin embedded (mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (USP28) Polyclonal Antibody, Unconjugated (SL5820R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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