Rabbit Anti-hnRNP F antibody

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Heterogeneous nuclear ribonucleoprotein F; Heterogeneous nuclear ribonucleoprotein F; hnRNP F; HNRNPF; hnRP F; hnRPF; mcs94 1; MGC110997; Nucleolin like protein; Nucleolin like protein mcs94 1; OK/SW cl.23; Ribonucleoprotein F; HNRPF_HUMAN.

Cat:

SL4205R

Species Reactivity：

Mouse,Rat,(predicted: Human,Dog,Pig,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human hnRNP F:301-415/415

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Tissue/cell: mouse pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-hnRNP F Polyclonal Antibody, Unconjugated(SL4205R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-hnRNP F Polyclonal Antibody, Unconjugated(SL4205R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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hnRNP F is part of the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). These are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA) and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. hnRNP F binds to RNAs which have guanosine-rich sequences.

Function:
Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state.

Subunit:
Identified in the spliceosome C complex. Interacts with AGO1, AGO2, TBP and TXNL4/DIM1.

Subcellular Location:
Nucleus, nucleoplasm.

Tissue Specificity:
Expressed ubiquitously.

Post-translational modifications:
Sumoylated.

Similarity:
Contains 3 RRM (RNA recognition motif) domains.

SWISS:
P52597

Gene ID:
3185

Database links:

Entrez Gene: 3185 Human

Entrez Gene: 98758 Mouse

Entrez Gene: 64200 Rat

Omim: 601037 Human

SwissProt: Q5E9J1 Cow

SwissProt: P52597 Human

SwissProt: Q9Z2X1 Mouse

SwissProt: Q794E4 Rat

Unigene: 712955 Human

Unigene: 808 Human

Unigene: 422979 Mouse

Unigene: 476420 Mouse

Unigene: 144596 Rat

Picture

Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-hnRNP F Polyclonal Antibody, Unconjugated(SL4205R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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