Has RNase activity and selectively degrades specific target mRNA species. Modulates the immune response and inflammation by regulating the decay of specific mRNA molecules. Recognizes the 3'-untranslated region (UTR) of the mRNA for IL6, CALCR and IL12B. Required for normal decay of IL6 mRNA (By similarity). Triggers apoptosis and promotes angiogenesis in response to the binding of CCL2 to CCR2. Regulates expression of CDH12 and CHD19.
Function:
Has RNase activity and selectively degrades specific target mRNA species. Modulates the immune response and inflammation by regulating the decay of specific mRNA molecules. Recognizes the 3'-untranslated region (UTR) of the mRNA for IL6, CALCR and IL12B. Required for normal decay of IL6 mRNA (By similarity). Triggers apoptosis and promotes angiogenesis in response to the binding of CCL2 to CCR2. Regulates expression of CDH12 and CHD19.
Subcellular Location:
Cytoplasm. Nucleus.
Similarity:
Belongs to the ZC3H12 family.
Contains 1 C3H1-type zinc finger.
SWISS:
Q5D1E8
Gene ID:
80149
Database links:
Entrez Gene: 535344 Cow
Entrez Gene: 80149 Human
Entrez Gene: 230738 Mouse
Omim: 610562 Human
SwissProt: A6QQJ8 Cow
SwissProt: Q5D1E8 Human
SwissProt: Q5D1E7 Mouse
Unigene: 656294 Human
Unigene: 402 Mouse
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Sample:
Raw264.7 Cell (Mouse) Lysate at 40 ug
Hela Cell (Human) Lysate at 40 ug
HepG2 Cell (Human) Lysate at 40 ug
Primary: Anti- MCPIP1 (SL5792R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 66 kD
Observed band size: 66 kD
k control (Black line): Hela (Black).
Primary Antibody (green line): Rabbit Anti-MCPIP1 antibody (SL5972R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at -20℃ .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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