Rabbit Anti-ZNF185 antibody

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LIM domain protein ZNF185; P1 A; Zinc finger protein 185 (LIM domain); ZN185_HUMAN.

Cat:

SL6119R

Species Reactivity：

Human,(predicted: Mouse,Rat,Dog,)

Immunogen：

KLH conjugated synthetic peptide derived from human ZNF185:551-650/689

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000Flow-Cyt=3ug/Testnot yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: MCF-7(Human) Cell Lysate at 40 ugA431(Human) Cell Lysate at 40 ugU2os(Human) Cell Lysate at 40 ugPrimary: Anti-ZNF185 (SL6119R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 76/39 kDObserved band size: 67 kDBlank control: A431. Primary Antibody (green line): Rabbit Anti-Phospho-c-Fos (Ser32) antibody (SL6199R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647Dilution: 1μg /test. ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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ZNF185 encodes a LIM domain zinc finger protein. It may be involved in the regulation of cellular proliferation and/or differentiation.

Function:
May be involved in the regulation of cellular proliferation and/or differentiation.

Subcellular Location:
Cytoplasm, cytoskeleton. Cell junction, focal adhesion.

Tissue Specificity:
Expressed in placenta, pancreas and kidney. Also expressed in prostate, testis, ovary and blood.

Similarity:
Contains 1 LIM zinc-binding domain.

SWISS:
O15231

Gene ID:
7739

Database links:

Entrez Gene: 7739 Human

Entrez Gene: 22673 Mouse

Omim: 300381 Human

SwissProt: O15231 Human

SwissProt: Q62394 Mouse

Unigene: 16622 Human

Unigene: 1161 Mouse

Picture

Blank control: A431.
Primary Antibody (green line): Rabbit Anti-Phospho-c-Fos (Ser32) antibody (SL6199R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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