Semaphorins are a large family, including both secreted and membrane associated proteins, many of which have been implicated as inhibitors or chemorepellents in axon pathfinding, fasciculation and branching, and target selection. All semaphorins possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Additional sequence motifs SLCterminal to the semaphorin domain allow classification into distinct subfamilies. Results demonstrate that transmembrane semaphorins, like the secreted ones, can act as repulsive axon guidance cues. This gene encodes a class 6 vertebrate transmembrane semaphorin that demonstrates alternative splicing. Several transcript variants have been identified and expression of the distinct encoded isoforms is thought to be regulated in a tissue- and development-dependent manner. [provided by RefSeq, Nov 2010]
Function:
Shows growth cone collapsing activity on dorsal root ganglion (DRG) neurons in vitro. May be a stop signal for the DRG neurons in their target areas, and possibly also for other neurons. May also be involved in the maintenance and remodeling of neuronal connections.
Subcellular Location:
Isoform 1: Cell membrane; Single-pass type I membrane protein.
Isoform 2: Cell membrane; Single-pass type I membrane protein.
Isoform 3: Cell membrane; Single-pass type I membrane protein.
Isoform 4: Cell membrane; Single-pass type I membrane protein.
Isoform 5: Cell membrane; Single-pass type I membrane protein.
Isoform 7: Cytoplasm.
Similarity:
Belongs to the semaphorin family.
Contains 1 PSI domain.
Contains 1 Sema domain.
SWISS:
Q8NFY4
Gene ID:
80031
Database links:
Entrez Gene: 100758662 Chinese Hamster
Entrez Gene: 518458 Cow
Entrez Gene: 100855988 Dog
Entrez Gene: 100715046 Guinea pig
Entrez Gene: 100055875 Horse
Entrez Gene: 80031 Human
Entrez Gene: 214968 Mouse
Entrez Gene: 100353114 Rabbit
Entrez Gene: 32284 Rat
Entrez Gene: 101112064 Sheep
Omim: 609295 Human
SwissProt: Q8NFY4 Human
SwissProt: Q76KF0 Mouse
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Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SEMA6D) Polyclonal Antibody, Unconjugated (SL2943R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SEMA6D) Polyclonal Antibody, Unconjugated (SL2943R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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