Rabbit Anti-LC3C antibody | Sunlong Medical

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LC3 like protein 2; MAP1 light chain 3 like protein 3; Microtubule-associated proteins 1A/1B light chain 3C; Autophagy-related protein LC3 C; Autophagy-related ubiquitin-like modifier LC3 C; MAP1 light chain 3-like protein 3; MAP1A/MAP1B light chain 3 C;

Cat:

SL6230R

Species Reactivity：

Human,Rat,Zebrafish,(predicted: Chicken,Dog,Pig,Cow,)

Immunogen：

KLH conjugated synthetic peptide derived from human LC3C:31-126/147

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Heart (Rat) Lysate at 40 ugPrimary: Anti-LC3C (SL6230R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 16 kDObserved band size: 18 kDParaformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LC3C) Polyclonal Antibody, Unconjugated (SL6230R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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A major contributor to cellular homeostasis is the ability of the cell to strike a balance between the formation and degradation/removal of its cellular components. This process of internal cellular turn-over is called autophagy (self-eating), and is facilitated by a pathway of around 16 interacting proteins in the human. LC3, a ubiquitin-like modifier protein, is the human homolog of yeast Apg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. There are three isoforms of human LC3 (named MAP1LC3A, MAP1LC3B, and MAP1LC3C), which exhibit different tissue distributions. A disruption to the autophagic process is now associated with the progression of several cancers, neurodegenerative disorders and cardiac pathologies, where LC3 is widely employed as a marker for autophagy.

Function:
Probably involved in formation of autophagosomal vacuoles (autophagosomes).

Subunit:
3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins.

Subcellular Location:
Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor.

Tissue Specificity:
Most abundant in placenta, lung and ovary.

Similarity:
Belongs to the MAP1 LC3 family.

SWISS:
Q9BXW4

Gene ID:
88738

Database links:

Entrez Gene: 88738 Human

Omim: 609605 Human

SwissProt: Q9BXW4 Human

Unigene: 534971 Human

Picture

Sample: Heart (Rat) Lysate at 40 ug
Primary: Anti-LC3C (SL6230R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 16 kD
Observed band size: 18 kD

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LC3C) Polyclonal Antibody, Unconjugated (SL6230R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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