Rabbit Anti-SQSTM1/P62 antibody

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SQSTM_HUMAN; Sequestosome-1; ORCA; OSIL; EBI3-associated protein of 60 kDa (EBIAP; p60); Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Ubiquitin-binding protein p62;

Cat:

SL2951R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Pig,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human SQSTM1/p62:51-150/440

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SQSTM1 P62) Polyclonal Antibody, Unconjugated (SL2951R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SQSTM1 P62) Polyclonal Antibody, Unconjugated (SL2951R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SQSTM1) Polyclonal Antibody, Unconjugated (SL2951R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-SQSTM1 Polyclonal Antibody, Unconjugated(SL2951R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingHepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (SQSTM1/P62) polyclonal Antibody, Unconjugated (SL2951R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009]

Function:
Adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels.

Subunit:
Cytoplasm. Late endosome. Nucleus. Endoplasmic reticulum. Note=Sarcomere. In cardiac muscles localizes to the sarcomeric band. Localizes to late endosomes. May also localize to the nucleus. Accumulates in neurofibrillary tangles and in Lewy bodies of neurons from individuals with Alzheimer and Parkinson disease respectively. Enriched in Rosenthal fibers of pilocytic astrocytoma. In liver cells, accumulates in Mallory bodies associated with alcoholic hepatitis, Wilson disease, indian childhood cirrhosis and in hyaline bodies associated with hepatocellular carcinoma. Colocalizes with TRIM13 in the perinuclear endoplasmic reticulum.

Subcellular Location:
Cytoplasm. Late endosome. Nucleus. Sarcomere. In cardiac muscles localizes to the sarcomeric band. Localizes to late endosomes. May also localize to the nucleus. Accumulates in neurofibrillary tangles and in Lewy bodies of neurons from individuals with Alzheimer and Parkinson disease respectively. Enriched in Rosenthal fibers of pilocytic astrocytoma. In liver cells, accumulates in Mallory bodies associated with alcoholic hepatitis, Wilson disease, indian childhood cirrhosis and in hyaline bodies associated with hepatocellular carcinoma.

Tissue Specificity:
Ubiquitously expressed.

Post-translational modifications:
Phosphorylated. May be phosphorylated by PRKCZ (By similarity). Phosphorylated in vitro by TTN.

DISEASE:
Defects in SQSTM1 are a cause of Paget disease of bone (PDB) [MIM:602080]. PDB is a metabolic bone disease affecting the axial skeleton and characterized by focal areas of increased and disorganized bone turn-over due to activated osteoclasts. Manifestations of the disease include bone pain, deformity, pathological fractures, deafness, neurological complications and increased risk of osteosarcoma. PDB is a chronic disease affecting 2 to 3% of the population above the age of 40 years.

Similarity:
Contains 1 OPR domain.
Contains 1 UBA domain.
Contains 1 ZZ-type zinc finger.

SWISS:
Q13501

Gene ID:
8878

Database links:

Entrez Gene: 8878 Human

Entrez Gene: 18412 Mouse

Entrez Gene: 22894 Rat

SwissProt: Q13501 Human

SwissProt: Q64337 Mouse

SwissProt: O08623 Rat

Picture

Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SQSTM1 P62) Polyclonal Antibody, Unconjugated (SL2951R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SQSTM1) Polyclonal Antibody, Unconjugated (SL2951R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.

Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-SQSTM1 Polyclonal Antibody, Unconjugated(SL2951R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (SQSTM1/P62) polyclonal Antibody, Unconjugated (SL2951R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Product Feedback Wall

Specific References (19) | SL2951R has been referenced in 19 publications.

[IF=2.751] Jiachen Lv. et al. Alcohol Aggravates Acute Pancreatitis by Impairing Autophagic Flux Through Activation of AMPK Signaling Pathway. 2021 Feb 08 WB,IHC ; Human,Rat.

PubMed:33555515

[IF=1.698] Sheng-Tao Ling. et al. Hydroxychloroquine Blocks Autophagy and Promotes Apoptosis of the Prostate after Castration in Rats. Urol Int. 2020;104(11-12):968-974 IHC ; Rat.

PubMed:32937616

[IF=3.067] Kang Z et al. Copper-induced apoptosis and autophagy through oxidative stress-mediated mitochondrial dysfunction in male germ cells. Toxicol In Vitro. 2019 Sep 3;61:104639. WB ; Mouse.

PubMed:3149296

[IF=2.107] Yin X et al. Lithium facilitates removal of misfolded proteins and attenuated faulty interaction between mutant SOD1 and p-CREB (Ser133) through enhanced autophagy in mutant hSOD1G93A transfected neuronal cell lines. Mol Biol Rep. 2019 Sep 16. WB ; Human.

PubMed:31529340

[IF=2.798] Kim J et al. Human β-defensin 2 is involved in CCR2-mediated Nod2 signal transduction, leading to activation of the innate immune response in macrophages. Immunobiology. 2019 May 18. WB ; Human.

PubMed:31126693

[IF=3.68] Yang and Yao JNK-Bcl-2/Bcl-xL-Bax/Bak Pathway Mediates the Crosstalk between Matrine-Induced Autophagy and Apoptosis via Interplay with Beclin 1. (2015) Int.J.Mol.Sci. 16:25744-58 WB ; Human.

PubMed:26516844

[IF=2.82] Cuellar et al. Immunohistochemical Localization of Bone Morphogenetic Proteins (BMPs) and their Receptors in Solitary and Multiple Human Osteochondromas. (2014) J.Histochem.Cytochem. 62:488-498 IHC ; Human.

PubMed:24781964

[IF=2.78] Chen, Yunbo, et al. "β-Asarone prevents autophagy and synaptic loss by reducing ROCK expression in asenescence-accelerated prone 8 mice." Brain research 1552 (2014): 41-54. WB ; Mouse.

PubMed:24457043

[IF=0] Xia, Xj, et al. "Autophagy mediated by arginine depletion activation of the nutrient sensor GCN2 contributes to interferon-γ-induced malignant transformation of primary bovine mammary epithelial cells." Cell Death Discovery 2 (2016). IHSLCP ; Bovine.

PubMed:27551491

[IF=2.19] Cui, Xiaodong, et al. "Changes of intracellular Ca2+ in quercetin-induced autophagy progression." Acta Biochimica et Biophysica Sinica (2015): gmv096. WB ; Human.

PubMed:26423114

[IF=5.74] Kuo, Hsiao-Mei, et al. "Altered Mitochondrial Dynamics and Response to Insulin in Cybrid Cells Harboring a Diabetes-susceptible Mitochondrial DNA Haplogroup." Free Radical Biology and Medicine (2016). WB ; Human.

PubMed:27107769

[IF=15.2] Lovren, Fina, et al. "MicroRNA-145 Targeted Therapy Reduces Atherosclerosis." Circulation 126.11 suppl 1 (2012): S81-S90. IHSLCF ; Mouse.

PubMed:22965997

[IF=2.613] Kong Q et al. Autophagy inhibits TLR4‐mediated invasiveness of oral cancer cells via the NF‐κB pathway. Oral Dis. 2020 Apr 14. WB&Co-IP ; Human.

PubMed:32291890

[IF=3.647] Lin D et al. Aldehyde dehydrogenase 2 regulates autophagy via the Akt-mTOR pathway to mitigate renal ischemia-reperfusion injury in hypothermic machine perfusion. Life Sci . 2020 Jul 15;253:117705. IHC,WB ; mouse.

PubMed:32334008

[IF=2.613] Kong Q et al. Autophagy Inhibits TLR4‐mediated Invasiveness of Oral cancer Cells Via the NF‐κB Pathway. Oral Dis . 2020 Apr 14. WB ; human.

PubMed:32291890

[IF=3.414] Ma HX et al. Mu-Xiang-You-Fang protects PC12 cells against OGD/R-induced autophagy via the AMPK/mTOR signaling pathway. J Ethnopharmacol. 2020 Jan 22;252:112583. ICF&WB ; Rattus norvegicus.

PubMed:31978519

[IF=2.819] Wang B et al. Resolvin D1 Resolve Inflammation in Experimental Acute Pancreatitis by Restoring Autophagic Flux.Dig Dis Sci. 2018 Dec;63(12):3359-3366. WB ; Mouse.

PubMed:29974378

[IF=3.166] Wang Q et al. PCB28 and PCB52 induce hepatotoxicity by impairing the autophagic flux and stimulating cell apoptosis in vitro. Toxicol Lett. 2018 Jun 1;289:28-41. WB&IF ; Human &Rat.

PubMed:29518472

[IF=3.257] Wang et al. Blocking autophagic flux enhances matrine-induced apoptosis in human hepatoma cells. (2013) Int.J.Mol.Sc. 14:23212-30 WB ; Human.

PubMed:24287900