Rabbit Anti-Desmoglein 3 antibody

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130 kD pemphigus vulgaris antigen; Balding; CDHF 6; CDHF6; Desmoglein 3 precursor; Desmoglein3; DKFZp686P23184; DSG 3; DSG3; DSG-3; Pemphigus vulgaris antigen; PVA; DSG3_HUMAN.

Cat:

SL6585R

Species Reactivity：

Mouse,Rat,(predicted: Human,Dog,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human Desmoglein 3:50-75/999<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Tissue/cell: rat tongue tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Desmoglein 3 Polyclonal Antibody, Unconjugated(SL6585R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmoglein 3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This protein has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris.

Subcellular Location:
Cell membrane; Single-pass type I membrane protein. Cell junction, desmosome.

Tissue Specificity:
Epidermis, tongue, tonsil, esophagus and carcinomas.

Similarity:
Contains 4 cadherin domains.

SWISS:
P32926

Gene ID:
1830

Database links:

Entrez Gene: 1830 Human

Omim: 169615 Human

SwissProt: P32926 Human

Unigene: 1925 Human

Picture

Tissue/cell: rat tongue tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Desmoglein 3 Polyclonal Antibody, Unconjugated(SL6585R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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