Rabbit Anti-RNF43 antibody | Sunlong Medical

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RNF124; Ring finger protein 43; URCC; RNF43_HUMAN; E3 ubiquitin-protein ligase RNF43; RING finger protein 43.

Cat:

SL7007R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human RNF43:41-140/783

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ugLane 2: Kidney (Mouse) Lysate at 40 ugLane 3: Heart (Mouse) Lysate at 40 ugLane 4: Cerebrum (Rat) Lysate at 40 ugLane 5: Kidney (Rat) Lysate at 40 ugPrimary: Anti-RNF43 (SL7007R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 80 kDObserved band size: 83 kDSample: Embryo (Mouse) Lysate at 40 ugPrimary: Anti-RNF43 (SL7007R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 83 kDObserved band size: 93 kDTissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-RNF43 Polyclonal Antibody, Unconjugated(SL7007R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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RNF43 is a probable E3 ubiquitin-protein ligase that promotes cell growth and is upregulated in colon cancer. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.

Function:
Probable E3 ubiquitin-protein ligase. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.

Subunit:
Interacts with AKAP8L, NONO and SFPQ.

Subcellular Location:
Endoplasmic reticulum membrane; Single-pass type I membrane protein (Potential). Nucleus envelope.

Tissue Specificity:
Expressed in fetal kidney, fetal lung, in colon cancer tissues, hepatocellular carcinomas and lung adenocarcinomas. Overexpressed in colorectal cancer cell lines.

Similarity:
Contains 1 RING-type zinc finger.

SWISS:
Q68DV7

Gene ID:
54894

Database links:

Entrez Gene: 54894 Human

Entrez Gene: 207742 Mouse

Entrez Gene: 303412 Rat

Omim: 612482 Human

SwissProt: Q68DV7 Human

SwissProt: Q5NCP0 Mouse

Unigene: 584916 Human

Unigene: 88230 Mouse

Unigene: 214174 Rat

Picture

Sample:
Embryo (Mouse) Lysate at 40 ug
Primary: Anti-RNF43 (SL7007R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 83 kD
Observed band size: 93 kD

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-RNF43 Polyclonal Antibody, Unconjugated(SL7007R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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