Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. Signaling by Wnt-7a allows sexually dimorphic development of the mullerian ducts.
Function:
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. Signaling by Wnt-7a allows sexually dimorphic development of the mullerian ducts (By similarity).
Subunit:
Interacts with PORCN.
Subcellular Location:
Secreted, extracellular space, extracellular matrix.
Tissue Specificity:
Expression is restricted to placenta, kidney, testis, uterus, fetal lung, and fetal and adult brain.
DISEASE:
Defects in WNT7A are the cause of limb pelvis hypoplasia aplasia syndrome (LPHAS) [MIM:276820]. A syndrome of severe deficiency of the extremities due to hypo- or aplasia of one or more long bones of one or more limbs. Pelvic manifestations include hip dislocation, hypoplastic iliac bone and aplastic pubic bones. Thoracic deformity, unusual facies and genitourinary anomalies can be present.
Defects in WNT7A are a cause of Fuhrmann syndrome (FUHRS) [MIM:228930]; also known as fibular aplasia or hypoplasia femoral bowing and poly- syn- and oligodactyly. Fuhrmann syndrome is a distinct limb-malformation disorder characterized also by various degrees of limb aplasia/hypoplasia and joint dysplasia.
Similarity:
Belongs to the Wnt family.
SWISS:
O00755
Gene ID:
7476
Database links:
Entrez Gene: 7476 Human
Entrez Gene: 533782 Cow
Entrez Gene: 60736 Dog
Entrez Gene: 100055450 Horse
Entrez Gene: 22421 Mouse
Entrez Gene: 100355697 Rabbit
Entrez Gene: 114850 Rat
Omim: 601570 Human
SwissProt: O00755 Human
SwissProt: P24383 Mouse
Unigene: 72290 Human
Unigene: 56964 Mouse
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Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-WNT7A (SL6645R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 50 kD
Sample:
Lane 1: Mouse Placenta tissue lysates
Lane 2: Mouse Lung tissue lysates
Lane 3: Mouse Testis tissue lysates
Lane 4: Mouse Kidney tissue lysates
Lane 5: Rat Placenta tissue lysates
Lane 6: Rat Lung tissue lysates
Lane 7: Rat Testis tissue lysates
Lane 8: Rat Kidney tissue lysates
Lane 9: Human Hela cell lysates
Lane 10: Human A431 cell lysates
Lane 11: Human SH-SY5Y cell lysates
Lane 12: Human HepG2 cell lysates
Primary: Anti- WNT7A (SL6645R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WNT7A) Polyclonal Antibody, Unconjugated (SL6645R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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