Sample:
Lane 1: Lung (Mouse) Lysate at 40 ug
Lane 2: Lung (Rat) Lysate at 40 ug
Lane 3: U87MG (Human) Lysate at 40 ug
Lane 4: Hela (Human) Lysate at 40 ug
Primary: Anti-ApoER2 (SL6651R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 170/130 kD
Observed band size: 130/125 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-ApoER2 Polyclonal Antibody, Unconjugated(SL6651R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining