Rabbit Anti-GAS 6 antibody | Sunlong Medical

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AXL receptor tyrosine kinase ligand; AXL stimulatory factor; AXLLG; AXS F; AXSF; Gas 6; GAS6; Growth-arrest-specific protein 6; Growth-arrest-specific protein 6 precursor.

Cat:

SL7549R

Species Reactivity：

Human,Mouse,(predicted: Rat,Chicken,Dog,Pig,Cow,Horse,)

Immunogen：

KLH conjugated synthetic peptide derived from human GAS 6:551-650/721

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Liver (Mouse) Lysate at 40 ugKidney (Mouse) Lysate at 40 ugPrimary: Anti-GAS 6 (SL7549R) at 1/300 dilutionSecondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilutionPredicted band size: 76 kDObserved band size: 76 kDTissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-GAS6 Polyclonal Antibody, Unconjugated(SL7549R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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GAS 6 is a ligand for the tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS 6 regulates chondrogenic differentiation from mesenchymal cells and also plays a role in thrombosis by amplifying platelet aggregation and secretion in response to known agonists. It is over expressed in human gliomas and regulate the phagocytic function of sertoli cells.

Function:
Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses.

Subunit:
Heterodimer and heterotetramer with AXL.

Subcellular Location:
Secreted.

Tissue Specificity:
Plasma. Isoform 1 and isoform 2 are widely expressed. Isoform 1 is the predominant form in spleen.

Post-translational modifications:
Isoform 1 is proteolytically processed after secretion to yield a N-terminal 36 kDa protein and a SLCterminal 50 kDa protein including the laminin G-like domains which activates AXL.
Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.

Similarity:
Contains 4 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 2 laminin G-like domains.

SWISS:
Q14393

Gene ID:
2621

Database links:

Entrez Gene: 2621 Human

Entrez Gene: 14456 Mouse

Omim: 600441 Human

SwissProt: Q14393 Human

SwissProt: Q61592 Mouse

Unigene: 646346 Human

Unigene: 3982 Mouse

GAS6在基因转录调节中起重要的调节作用,参与对细胞存活、增殖、粘附、迁移及抗凋亡的调节，Axl属于受体酪氨酸激酶(receptor tyrosine kinases,RTK)家族成员,为Axl的生物配体。经研究发现；Gas6与多种肿瘤细胞的增殖、存活、血管发生、侵袭有关，Gas6系统具有广泛的生物学作用，Gas6属维生素K依赖的蛋白质,广泛表达于各类细胞。 Picture

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-GAS6 Polyclonal Antibody, Unconjugated(SL7549R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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