PEF1 (penta-EF hand domain containing 1) is a Ca(2+)-binding protein that belongs to the penta-EF hand family which shares similarities to the apoptosis linked gene ALG-2. PEF1 heterodimerizes with PDCD6/ALG2 and dissociates from PDCD6/ALG2 in the presence of Ca(2+).
Subunit:
Heterodimer; heterodimerizes with PDCD6. Dissociates from PDCD6 in presence of Ca(2+).
Subcellular Location:
Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated in the presence of Ca(2+).
Similarity:
Contains 5 EF-hand domains.
SWISS:
Q9UBV8
Gene ID:
553115
Database links:
Entrez Gene: 553115 Human
Entrez Gene: 67898 Mouse
Entrez Gene: 297900 Rat
Omim: 610033 Human
SwissProt: Q9UBV8 Human
SwissProt: Q8SFY6 Mouse
SwissProt: Q641Z8 Rat
Unigene: 470417 Human
Unigene: 20818 Mouse
Unigene: 96097 Rat
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Sample: Intestine (Mouse) Lysate at 40 ug
Primary: Anti-PEF1 (SL6768R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 30 kD
Blank control: Hela(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.
Isotype Control Antibody: Rabbit IgG(orange) ;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue),
Dilution: 1:200 in 1 X PBS containing 0.5% BSA ;
Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).
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