Rabbit Anti-E1 Ubiquitin Activating Enzyme antibody

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Uba1; A1S9; A1S9 protein; A1S9T and BN75 temperature sensitivity complementing; A1S9T; A1ST; GXP 1; GXP1; MGC4781; Protein A1S9; UBA1_HUMAN; UBE 1 ; UBE 1X; UBE1; UBE1X; Ubiquitin activating enzyme E1; Ubiquitin-activating enzyme E1; Ubiquitin-like modifi

Cat:

SL6835R

Species Reactivity：

Human,Rat,(predicted: Mouse,Dog,Pig,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human UBE1:961-1058/1058

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (E1 Ubiquitin Activating Enzyme) Polyclonal Antibody, Unconjugated (SL6835R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Blank control(blue):293T Cells(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-E1 Ubiquitin Activating Enzyme/AF488 Conjugated antibody (SL6835R-AF488), Dilution: 1ug in 100 ul 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq, Jul 2008].

Function:
Activates ubiquitin by first adenylating its SLCterminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.

Subunit:
Monomer (By similarity). Interacts with GAN (via BTB domain).

Post-translational modifications:
ISGylated.

DISEASE:
Defects in UBA1 are the cause of spinal muscular atrophy X-linked type 2 (SMAX2) [MIM:301830]; also known as X-linked lethal infantile spinal muscular atrophy, distal X-linked arthrogryposis multiplex congenita or X-linked arthrogryposis type 1 (AMCX1). Spinal muscular atrophy refers to a group of neuromuscular disorders characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. SMAX2 is a lethal infantile form presenting with hypotonia, areflexia, and multiple congenital contractures.

Similarity:
Belongs to the ubiquitin-activating E1 family.

SWISS:
P22314

Gene ID:
7317

Database links:

Entrez Gene: 7317 Human

Entrez Gene: 22201 Mouse

Entrez Gene: 314432 Rat

Omim: 314370 Human

SwissProt: P22314 Human

SwissProt: Q02053 Mouse

SwissProt: Q5U300 Rat

Unigene: 533273 Human

Unigene: 1104 Mouse

Unigene: 474674 Mouse

Unigene: 2360 Rat

Picture

Blank control(blue):293T Cells(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice).
Primary Antibody: Rabbit Anti-E1 Ubiquitin Activating Enzyme/AF488 Conjugated antibody (SL6835R-AF488), Dilution: 1ug in 100 ul 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions.

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