Rabbit Anti-STAMBP antibody

Home > Product > Antibody > Rabbit Anti-STAMBP antibody

AMSH; Associated molecule with the SH3 domain of STAM; Endosome associated ubiquitin isopeptidase; Endosome-associated ubiquitin isopeptidase; STABP_HUMAN; STAM binding protein; STAM-binding protein; STAMBP.

Cat:

SL7735R

Species Reactivity：

Mouse,Rat,(predicted: Human,Dog,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human AMSH:331-420/424

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AMSH) Polyclonal Antibody, Unconjugated (SL7735R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-AMSH/STAMBP Polyclonal Antibody, Unconjugated(SL7735R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

prev next

Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

Zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not cleave 'Lys-48'-linked polyubiquitin chains (By similarity). Functions at the endosome and is able to oppose the ubiquitin-dependent sorting of receptors to lysosomes. Plays a role in signal transduction for cell growth and MYC induction mediated by IL-2 and GM-CSF. Potentiates BMP (bone morphogenetic protein) signaling by antagonizing the inhibitory action of SMAD6 and SMAD7.

Function:
Zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not cleave 'Lys-48'-linked polyubiquitin chains (By similarity). Functions at the endosome and is able to oppose the ubiquitin-dependent sorting of receptors to lysosomes. Plays a role in signal transduction for cell growth and MYC induction mediated by IL-2 and GM-CSF. Potentiates BMP (bone morphogenetic protein) signaling by antagonizing the inhibitory action of SMAD6 and SMAD7.

Subcellular Location:
Nucleus. Membrane; Peripheral membrane protein. Cytoplasm. Early endosome.

Tissue Specificity:
Ubiquitously expressed.

Post-translational modifications:
Phosphorylated after BMP type I receptor activation.
Ubiquitinated by SMURF2 in the presence of RNF11.

Similarity:
Belongs to the peptidase M67C family.
Contains 1 MPN (JAB/Mov34) domain.

SWISS:
O95630

Gene ID:
10617

Database links:

Entrez Gene: 10617 Human

Entrez Gene: 70527 Mouse

Entrez Gene: 171565 Rat

Omim: 606247 Human

SwissProt: O95630 Human

SwissProt: Q9CQ26 Mouse

SwissProt: Q8R424 Rat

Unigene: 469018 Human

Unigene: 657598 Human

Unigene: 32801 Mouse

Unigene: 14662 Rat

Picture

Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-AMSH/STAMBP Polyclonal Antibody, Unconjugated(SL7735R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Product Feedback Wall